Responses of the Antioxidant System in QGY-7701 Cells to the Cytotoxicity and Apoptosis Induced by 1-Octyl-3-methylimidazolium Chloride

Authors


  • Contract grant sponsor: National Science Foundation of China.

  • Contract grant number: 31172415 and 20573019.

  • Contract grant sponsor: Science and Technology Foundation of Henan Province, China.

  • Contract grant number: 122102310195 and 12A180022.

Correspondence to: Xiaoyu Li.

ABSTRACT

This study aims to evaluate the cytotoxicity and responses of the cellular antioxidant system of 1-octyl-3-methylimidazolium chloride ([C8mim][Cl]) on human hepatocarcinoma QGY-7701 cells. The results show that [C8mim][Cl] can inhibit QGY-7701 cell growth and decrease their viabilities in a dose-dependent manner. The results also reveal that [C8mim][Cl] exposure can induce apoptosis in the [C8mim][Cl]-treated QGY-7701 cells. In addition, the results of biochemical assays show that [C8mim][Cl] exposure causes overproduction of reactive oxygen species (ROS), inhibits superoxide dismutase (SOD) and catalase (CAT) activities, decreases reduced glutathione (GSH) content, and increases the cellular malondialdehyde (MDA) level. These results suggest that ROS-mediated oxidative stress may be responsible for the apoptosis induced by [C8mim][Cl] in QGY-7701 cells. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:330-336, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21495

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