The author declares that there is no conflict of interest.
Benfotiamine Enhances Antioxidant Defenses and Protects against Cisplatin-Induced DNA Damage in Nephrotoxic Rats
Version of Record online: 28 MAY 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Volume 27, Issue 8, pages 398–405, August 2013
How to Cite
Harisa, G. I. (2013), Benfotiamine Enhances Antioxidant Defenses and Protects against Cisplatin-Induced DNA Damage in Nephrotoxic Rats. J. Biochem. Mol. Toxicol., 27: 398–405. doi: 10.1002/jbt.21501
- Issue online: 1 AUG 2013
- Version of Record online: 28 MAY 2013
- Manuscript Accepted: 3 MAY 2013
- Manuscript Revised: 22 APR 2013
- Manuscript Received: 26 FEB 2013
- nitric oxide;
The objective of the present study was to assess superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), paraoxonase (PON1), glutathione reductase (GR), and catalase (CAT) activities ratio and their relationship with DNA oxidative damage in rats treated with cisplatin (3 mg/kg bwt/day) in the presence and absence of benfotiamine (100 mg/kg/day) for 25 days. Cisplatin-induced renal damage was evidenced by renal dysfunction and elevated oxidative stress markers. SOD activity and levels of nitric oxide, protein carbonyl, malondialdehyde, and 8-hydroxy-2'-deoxyguanosine were significantly increased by cisplatin treatment. Moreover, the ratios of GPx/GR, SOD/GPx, SOD/CAT, and SOD/PON1 were significantly increased compared to control. In contrast, glutathione levels were significantly decreased by cisplatin treatment. Simultaneous treatment of rats with cisplatin and benfotiamine ameliorate these variables to values near to those of control rats. This study suggests that benfotiamine can prevent cisplatin-induced nephrotoxicity by inhibiting formation reactive species of oxygen and nitrogen. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:398-405, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21501