Contract Grant Sponsor: Interdisciplinary Programs in Education and Project in Research Development (P&P), KyushuUniversity, Japan.
Thiobencarb Herbicide Reduces Growth, Photosynthetic Activity, and Amount of Rieske Iron-Sulfur Protein in the Diatom Thalassiosira pseudonana
Article first published online: 25 JUN 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Volume 27, Issue 9, pages 437–444, September 2013
How to Cite
Shimasaki, Y., Tsuyama, M., Tasmin, R., Qiu, X., Shimizu, M., Sato, Y., Yamasaki, Y., Kato-Unoki, Y., Nukata, A., Nakashima, T., Ichinose, H., Wariishi, H., Honjo, T. and Oshima, Y. (2013), Thiobencarb Herbicide Reduces Growth, Photosynthetic Activity, and Amount of Rieske Iron-Sulfur Protein in the Diatom Thalassiosira pseudonana. J. Biochem. Mol. Toxicol., 27: 437–444. doi: 10.1002/jbt.21505
Contract Grant Sponsor: Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University, Japan.
Contract Grant Sponsor: Chemical Evaluation and Research Institute (CERI), Japan.
- Issue published online: 4 SEP 2013
- Article first published online: 25 JUN 2013
- Manuscript Accepted: 31 MAY 2013
- Manuscript Received: 22 APR 2013
- Interdisciplinary Programs in Education and Project in Research Development (P&P), Kyushu University
- Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University
- Chemical Evaluation and Research Institute (CERI)
- Thalassiosira pseudonana;
- Rieske Iron-Sulfur Protein
We investigated the effects of the herbicide thiobencarb on the growth, photosynthetic activity, and expression profile of photosynthesis-related proteins in the marine diatom Thalassiosira pseudonana. Growth rate was suppressed by 50% at a thiobencarb concentration of 1.26 mg/L. Growth and photosystem II activity (Fv/Fm ratio) were drastically decreased at 5 mg/L, at which the expression levels of 13 proteins increased significantly and those of 11 proteins decreased significantly. Among these proteins, the level of the Rieske iron-sulfur protein was decreased to less than half of the control level. This protein is an essential component of the cytochrome b6f complex in the photosynthetic electron transport chain. Although the mechanism by which thiobencarb decreased the Rieske iron-sulfur protein level is not clear, these results suggest that growth was inhibited by interruption of the photosynthetic electron transport chain by thiobencarb. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:437-444, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21505