Contract Grant Sponsor: Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil.
The Cytotoxic and Proapoptotic Activities of Hypnophilin are Associated with Calcium Signaling in UACC-62 Cells
Article first published online: 18 JUL 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Volume 27, Issue 11, pages 479–485, November 2013
How to Cite
Pinto, M. C. X., Cota, B. B., Rodrigues, M. A., Leite, M. F. and de Souza-Fagundes, E. M. (2013), The Cytotoxic and Proapoptotic Activities of Hypnophilin are Associated with Calcium Signaling in UACC-62 Cells. J. Biochem. Mol. Toxicol., 27: 479–485. doi: 10.1002/jbt.21507
Contract Grant Sponsor: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil.
Contract Grant Sponsor: Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brazil.
- Issue published online: 1 NOV 2013
- Article first published online: 18 JUL 2013
- Manuscript Accepted: 15 JUN 2013
- Manuscript Revised: 31 MAY 2013
- Manuscript Received: 6 APR 2013
- Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brazil
- Natural Products;
- Cancer Cells;
- Calcium Signaling
Hypnophilin (HNP) is a sesquiterpene that is isolated from Lentinus cf. strigosus and has cytotoxic activities. Here, we studied the calcium signaling and cytotoxic effects of HNP in UACC-62 cells, a human skin melanoma cell line. HNP was able to increase the intracellular calcium concentration in UACC-62 cells, which was blocked in cells stimulated in Ca2+-free media. HNP treatment with BAPTA-AM, an intracellular Ca2+ chelator, caused an increase in calcium signals. HNP showed cytotoxicity against UACC-62 cells in which it induced DNA fragmentation and morphological alterations, including changes in the nuclear chromatin profile and increased cytoplasmatic vacuolization, but it had no effect on the plasma membrane integrity. These data suggest that cytotoxicity in UACC-62 cells, after treatment with HNP, is associated with Ca2+ influx. Together, these findings suggest that HNP is a relevant tool for the further investigation of new anticancer approaches. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:479-485, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21507