A Comparative Study of In Vitro Cytotoxic, Antioxidant, and Antimicrobial Activity of Pt(II), Zn(II), Cu(II), and Co(III) Complexes with N-heteroaromatic Schiff Base (E)-2-[N′-(1-pyridin-2-yl-ethylidene)hydrazino]acetate
Article first published online: 3 DEC 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Volume 28, Issue 3, pages 99–110, March 2014
How to Cite
Filipović, N. R., Marković, I., Mitić, D., Polović, N., Milčić, M., Dulović, M., Jovanović, M., Savić, M., Nikšić, M., Anđelković, K. and Todorović, T. (2014), A Comparative Study of In Vitro Cytotoxic, Antioxidant, and Antimicrobial Activity of Pt(II), Zn(II), Cu(II), and Co(III) Complexes with N-heteroaromatic Schiff Base (E)-2-[N′-(1-pyridin-2-yl-ethylidene)hydrazino]acetate. J. Biochem. Mol. Toxicol., 28: 99–110. doi: 10.1002/jbt.21541
- Issue published online: 4 MAR 2014
- Article first published online: 3 DEC 2013
- Manuscript Accepted: 1 NOV 2013
- Manuscript Revised: 21 OCT 2013
- Manuscript Received: 17 SEP 2013
- Ministry of Education, Science and Technological Development of the Republic of Serbia. Grant Numbers: OI 172055, III 41025
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
Supporting information associated with the manuscript contain schematic representation of the ligand aphaOEt and Pt(II) complex 1, tabular representation of the substrates used for growing of microorganisms, 1H, 13C (experimental and calculated) and 15N NMR spectral data (derived from 1H–15N HMBC and HSQC spectra) of the ligand aphaOEt and the complex 1, the log sigmoid dose–response curves of free radical scavenging activity of the complexes 1–4, electrophoregram of plasmid pET20b form treated with in situ generated OH• radical in the presence or absence of the complexes, and experimental procedure and results of antimicrobial activity evaluation for the complexes and standard antimicotic/antibiotics. They can be obtained from the corresponding author upon request.
Scheme S1. Structures of aphaOEt and Pt(II) complex 1 along with atom numbering scheme. Figure S1. The log sigmoid dose–response curves of free radical scavenging activity of the complexes 1–4.
Figure S2. Electrophoregram of OC, Lin, and SC form of plasmid pET20b treated with in situ generated hydroxyl radical in the presence or absence of the complexes (Legend: c = DNA control; c* = DNA + H2O2 + UV; 1 = DNA + 1 + H2O2 + UV; 2 = DNA + 2 + H2O2 + UV; 3 = DNA + 3 + H2O2 + UV; 4 = DNA + 4 + H2O2 + UV).
Figure S3. Representative antimicrobial activity plots for the complexes 1–4 and standard antimicotic/antibiotics. The following antibiotics were used as standards: nystatin (all fungi strains), penicillin (L. monocytogenes and E. faecalis), chloramphenicol (B. cereus, G. stearothermophilus and Y. enterocolitic), gentamycin (E. coli O157:H7, S. enteritidis, and S. sonnei), amoxycyclin (P. aeruginosa and P. hauseri), and ampicillin (S. aureus).
Table S2. 1H and 13C NMR spectral data of the ligand aphaOEt and the complex 1.
Table S3. 15N NMR spectral data (derived from 1H–15N HMBC and HSQC spectra) of the ligand aphaOEt and the complex 1.
Table S4. Antimicrobial activities of the investigated compounds tested by the disc-diffusion method (inhibition zone size including disc, mm).
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