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p38 and Extracellular Signal-Regulated Kinases Activations have Opposite Effects on Primary-Cultured Rat Cerebellar Granule Neurons Exposed to Sodium Arsenite

Authors

  • Xiaona Liu,

    1. Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin, People's Republic of China
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  • Yanhui Gao,

    1. Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin, People's Republic of China
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  • Hongju Yao,

    1. Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin, People's Republic of China
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  • Lingwang Zhou,

    1. Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin, People's Republic of China
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  • Junrui Pei,

    1. Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin, People's Republic of China
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  • Liyan Sun,

    1. Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin, People's Republic of China
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  • Jing Wang,

    Corresponding author
    1. Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin, People's Republic of China
    • Correspondence to: Jing Wang and Dianjun Sun

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  • Dianjun Sun

    Corresponding author
    1. Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin, People's Republic of China
    • Correspondence to: Jing Wang and Dianjun Sun

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  • Jing Wang and Dianjun Sun contributed equally to the work.

  • Contract Grant Sponsor: National Natural Science Foundation of China.

  • Contract Grant Number: 81102082.

  • Contract Grant Sponsor: Natural Science Foundation of Heilongjiang Province of China

  • Contract Grant Number: D201053.

ABSTRACT

Arsenic is a widespread environmental toxicant in the world and regarded as both a carcinogen and an anticarcinogen. The present study was designed to evaluate roles of mitogen-activated protein kinases in sodium arsenite-induced effects on primary-cultured rat cerebellar granule neurons (CGNs). Results revealed a decreased viability of the cells exposed to sodium arsenite (from 0 to 50 μM) in a dose-dependent manner. Annexin V-fluorescein isothiocyanate assay showed that apoptosis was obviously induced by arsenite treatment. High phosphorylation expressions of p38 and extracellular signal-regulated kinases (ERK1/2), but not of c-Jun N-terminal kinases were observed due to arsenite treatment by western blotting analysis. Furthermore, SB203580 (an inhibitor of p38) decreased the percentage of apoptotic cells whereas arsenite-stimulated toxicity was enhanced by U0126 (an inhibitor of ERK1/2). Taken together, these data suggest that p38 contributes to arsenite-induced apoptosis of rat CGNs, but ERK1/2 may involve in cell growth and survival.

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