Inhibition of Hypoxia Inducible Factor 1α by siRNA-Induced Apoptosis in Human Retinoblastoma Cells
Version of Record online: 23 MAY 2014
© 2014 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Volume 28, Issue 9, pages 394–399, September 2014
How to Cite
Gao, Y., Jing, M., Ge, R., Zhou, Z. and Sun, Y. (2014), Inhibition of Hypoxia Inducible Factor 1α by siRNA-Induced Apoptosis in Human Retinoblastoma Cells. J. Biochem. Mol. Toxicol., 28: 394–399. doi: 10.1002/jbt.21576
- Issue online: 4 SEP 2014
- Version of Record online: 23 MAY 2014
- Manuscript Accepted: 30 APR 2014
- Manuscript Revised: 17 APR 2014
- Manuscript Received: 12 FEB 2014
- Hypoxia Inducible Factor 1α;
- Y-79 Retinoblastoma Cells
Hypoxia, which activates the hypoxia inducible factor 1α (HIF-1α), is an essential feature of retinoblastoma (RB) and contributes to poor prognosis and resistance to conventional therapy. In this study, the effect of HIF-1α knockdown by small interfering RNA (siRNA) on cell proliferation, apoptosis, and apoptotic pathways of human Y-79 RB cells was first investigated. Exposure to hypoxia induced the increased expression of HIF-1α both in mRNA and protein levels. Then, knockdown of HIF-1α by siRNAHIF-1α resulted in inhibition of cell proliferation and induced cell apoptosis in human Y-79 RB cells under both normoxic and hypoxic conditions, with hypoxic conditions being more sensitive. Furthermore, knockdown of HIF-1α could enhance hypoxia-induced slight increase of Bax/Bcl-2 ratio and activate caspase-9 and caspase-3. These results together indicated that suppression of HIF-1α expression may be a promising strategy for the treatment of human RB in the future.