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CD34+ collection efficiency as a function of blood volumes processed in pediatric autologous peripheral blood stem cell collection

Authors

  • Leonid Dubrovsky,

    Corresponding author
    1. Division of Bone Marrow Transplantation, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
    • Division of Bone Marrow Transplantation, Children's National Medical Center, 111 Michigan Ave., NW, Washington DC 20010
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  • Edward C.C. Wong,

    1. Division of Laboratory Medicine, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
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  • Evelio Perez-Albuerne,

    1. Division of Bone Marrow Transplantation, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
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  • Brett Loechelt,

    1. Division of Bone Marrow Transplantation, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
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  • Naynesh Kamani,

    1. Division of Bone Marrow Transplantation, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
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  • Jane Sande,

    1. Division of Bone Marrow Transplantation, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
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  • Kathy Mintz,

    1. Division of Bone Marrow Transplantation, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
    2. William and Shirley Howard Stem Cell Processing Laboratory, George Washington School of Medicine and Health Sciences, Washington DC
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  • Wendy Paul,

    1. Division of Laboratory Medicine, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
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  • Naomi L.C. Luban,

    1. Division of Laboratory Medicine, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
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  • Brian R. Rood,

    1. Division of Oncology, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
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  • Terry Fry

    1. Division of Bone Marrow Transplantation, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington DC
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Abstract

Purpose:

To characterize the relationship between CD34+ collection efficiency and blood volumes processed in pediatric patients undergoing autologous peripheral blood stem cell (PBSC) collection.

Methods:

Retrospective 8-year (2001–2009) study of pediatric patients (n = 79) with neuroblastoma and central nervous system (CNS) tumors undergoing first day of autologous PBSC harvest using MNC program on the COBE Spectra (Caridian BCT, Lakewood, CO) was performed. Patients undergoing 0 to 2.9 BV (standard volume), 3 to 6 BV (large volume), and greater than 6 BV (ultra large volume) harvests were evaluated for CD34+ collection efficiency, diagnosis (neuroblastoma vs. nonneuroblastoma), disease type (primary vs. relapse), mobilization regimen, granulocyte colony stimulating factor (GCSF) dose, and apheresis complications.

Results:

CD34+ collection efficiencies (CE) for neuroblastoma patients were 67%, 50%, and 53% for standard (n = 14), large (n = 9), and ultra large (n = 5) volume harvests, respectively. Similarly, patients with nonneuroblastoma diagnoses had CD34+ CE of 63%, 55%, and 65% for low (n = 19), large (n = 27), and ultra large (n = 5) volume harvests, respectively. Weight, granulocyte colony stimulating factor (G-CSF) stimulation, type of mobilization, and apheresis complications (normalized by run time) were similar between the standard, large, and ultra large volume groups in patients with either neuroblastoma or nonneuroblastoma diagnoses.

Conclusions:

CD34+ collection efficiency in pediatric autologous PBSC collection on the first day of harvest does not decrease with higher numbers of blood volumes processed in patients with either neuroblastoma or nonneuroblastoma primary disease. These results indirectly indicate bone marrow CD34+ cell mobilization occurs with longer apheresis procedures in pediatric patients. J. Clin. Apheresis, 2011. © 2011 Wiley-Liss, Inc.

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