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Journal of Cellular Biochemistry

Proline-rich transcript of the brain (prtb) is a serum-responsive gene in osteoblasts and upregulated during adhesion

Authors

  • Dirk W. Sommerfeldt,

    1. Department of Orthopaedics, State University of New York at Stony Brook, Stony Brook, New York 11794-2580
    Current affiliation:
    1. University Hospital Hamburg, Department of Trauma and Reconstructive Surgery, Martinistrasse 52, 20246 Hamburg, Germany.
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  • Jizu Zhi,

    1. Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-2580
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  • Clinton T. Rubin,

    1. Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-2580
    2. Center for Biotechnology, State University of New York at Stony Brook, Stony Brook, New York 11794-2580
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  • Michael Hadjiargyrou

    Corresponding author
    1. Department of Orthopaedics, State University of New York at Stony Brook, Stony Brook, New York 11794-2580
    2. Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-2580
    • Department of Biomedical Engineering, Psychology A, 3rd Floor, State University of New York at Stony Brook, Stony Brook, NY 11794-2580.
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    • *

      M.H. works as a Assistant Professor.


Abstract

To characterize the temporal expression of genes that play a functional role during the process of osteoblast adhesion, we used differential display (DD-PCR) on mRNA isolated from attached vs. suspended osteoblasts. A 200-bp fragment displaying upregulated expression after 30 and 60 min adhesion was isolated, sequenced, and showed 97% homology to prtb, previously showed to be expressed in mouse brain. Northern analysis confirmed a two-fold increase in prtb message during adhesion to tissue culture polystyrene, both in the presence or absence of surface-adsorbed serum proteins. Serum stimulation alone was also able to induce prtb expression, although to a lesser extent, in suspension cells. Strong prtb expression was also detected in both brain and bone of adult rats. Furthermore, prtb expression analysis during MC3T3-E1 cell differentiation revealed high expression levels independent of proliferation (day 0–7), matrix maturation (day 7–14), and mineralization (day 14–31). Time course analysis of prtb expression during adhesion of sensitized osteoblasts to serum-protein coated surfaces showed robust mRNA expression at 5 min post-plating and a peak at 10 min. The two known serum-inducible immediate early genes c-fos and c-jun showed similar expression kinetics, with c-jun mRNA levels peaking at 15 min and c-fos at 20 min. Based on these data, we hypothesize that prtb may function as an immediate early, serum-responsive, and adhesion-inducible gene with possible involvement in processes such as cell cycle control, adhesion, and proliferation. J. Cell. Biochem. 84: 301–308, 2002. © 2001 Wiley-Liss, Inc.

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