Article

Computationally focusing the directed evolution of proteins

  1. Christopher A. Voigt1,*,
  2. Stephen L. Mayo2,
  3. Frances H. Arnold3,
  4. Zhen-Gang Wang3

Article first published online: 29 JAN 2002

DOI: 10.1002/jcb.10066

Issue

Journal of Cellular Biochemistry

Journal of Cellular Biochemistry

Supplement: Enhancing Drug Discovery and Development

Volume 84, Issue Supplement 37, pages 58–63, 2001

Additional Information

How to Cite

Voigt, C. A., Mayo, S. L., Arnold, F. H. and Wang, Z.-G. (2001), Computationally focusing the directed evolution of proteins. J. Cell. Biochem., 84: 58–63. doi: 10.1002/jcb.10066

Author Information

  1. 1

    Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, California

  2. 2

    Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, California

  3. 3

    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California

*Biochemistry and Molecular Biophysics, California Institute of Technology, Mail code 210-41, Pasadena, CA, 91125.

Publication History

  1. Issue published online: 29 JAN 2002
  2. Article first published online: 29 JAN 2002
  3. Manuscript Accepted: 19 SEP 2001
  4. Manuscript Received: 18 SEP 2001

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Keywords:

  • directed evolution;
  • recombination;
  • mutagenesis;
  • computational methods

Abstract

Directed evolution has proven to be a successful strategy for the modification of enzyme properties. To date, the preferred experimental procedure has been to apply mutations or crossovers randomly throughout the gene. With the emergence of powerful computational methods, it has become possible to develop focused combinatorial searches, guided by computer algorithms. Here, we describe several computational methods that have emerged to aid the optimization of mutant libraries, the targeting of specific residues for mutagenesis, and the design of recombination experiments. J. Cell. Biochem. Suppl. 37: 58–63, 2001. © 2002 Wiley-Liss, Inc.

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