Article

Medulloblastoma cell invasion is inhibited by green tea (−)epigallocatechin-3-gallate

  1. Anthony Pilorget1,2,
  2. Virginie Berthet2,
  3. José Luis2,
  4. Albert Moghrabi3,
  5. Borhane Annabi1,
  6. Richard Béliveau1,3,*

Article first published online: 7 OCT 2003

DOI: 10.1002/jcb.10667

Issue

Journal of Cellular Biochemistry

Journal of Cellular Biochemistry

Volume 90, Issue 4, pages 745–755, 1 November 2003

Additional Information

How to Cite

Pilorget, A., Berthet, V., Luis, J., Moghrabi, A., Annabi, B. and Béliveau, R. (2003), Medulloblastoma cell invasion is inhibited by green tea (−)epigallocatechin-3-gallate. Journal of Cellular Biochemistry, 90: 745–755. doi: 10.1002/jcb.10667

Author Information

  1. 1

    Laboratoire de Médecine Moléculaire, Centre de Cancérologie Charles Bruneau, Hôpital Sainte-Justine and Université du Québec à Montréal, Montreal, Quebec, Canada

  2. 2

    UMR CNRS 6032 Faculté de Pharmacie, 27, bd Jean Moulin, 13385 Marseille, Cedex 5, France

  3. 3

    Division of Hematology-Oncology, Centre de Cancérologie Charles Bruneau, Hôpital Sainte-Justine, Montreal, Quebec, Canada

*Laboratoire de Médecine Moléculaire, Centre de Cancérologie Charles Bruneau, Hôpital Sainte-Justine, 3175 Côte Sainte-Catherine, Montréal, Québec, Canada, H3T 1C5.

Publication History

  1. Issue published online: 20 OCT 2003
  2. Article first published online: 7 OCT 2003
  3. Manuscript Accepted: 4 AUG 2003
  4. Manuscript Received: 23 JUN 2003

Funded by

  • Cancer Research Society (to RB)
  • Association pour la Recherche sur le Cancer
  • Groupement des Entreprises Françaises dans la Lutte contre le Cancer
  • Ligue Nationale contre le Cancer (to JL)

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (193K)

Keywords:

  • cell adhesion;
  • migration;
  • integrin;
  • EGCG;
  • green tea;
  • medulloblastoma

Abstract

Epigallocatechin-3-gallate (EGCG), the major green tea polyphenol, can reach the brain following oral intake and could thus act as an anti-tumoral agent targeting several key steps of brain cancer cells invasive activity. Because integrin-mediated extracellular matrix recognition is crucial during the cell adhesion processes involved in carcinogenesis, we have investigated the effects of EGCG on different cellular integrins of the pediatric brain tumor-derived medulloblastoma cell line DAOY. Using flow cytometry, we report the levels of expression of several cell surface integrins in DAOY. These include high expression of α2, α3, and β1 integrins, as well as αv and β3 integrins. Moreover, we provide evidence that EGCG can antagonize DAOY cell migration specifically on collagen by increasing cell adhesive ability through specific gene and protein upregulation of the β1 integrin subunit. Our results suggest that this naturally occurring green tea polyphenol may thus be used as a nutraceutical therapeutic agent in targeting the invasive character of medulloblastomas. © 2003 Wiley-Liss, Inc.

View Full Article (HTML) Get PDF (193K)