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Journal of Cellular Biochemistry

Antiviral effect of Phyllanthus nanus ethanolic extract against hepatitis B virus (HBV) by expression microarray analysis

Authors

  • Wai-Yip Lam,

    1. Department of Biochemistry, The Croucher Laboratory for Human Genomics, The Chinese University of Hong Kong, Shatin, Hong Kong, China
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  • Kam-Tong Leung,

    1. Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China
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  • Patrick Tik-Wan Law,

    1. Department of Biochemistry, The Croucher Laboratory for Human Genomics, The Chinese University of Hong Kong, Shatin, Hong Kong, China
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  • Simon Ming-Yuen Lee,

    1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China
    2. Institute of Chinese Medical Science, University of Macau, Macao SAR, China
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  • Henry Lik-Yuen Chan,

    1. Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, Hong Kong, China
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  • Kwok-Pui Fung,

    1. Department of Biochemistry, The Croucher Laboratory for Human Genomics, The Chinese University of Hong Kong, Shatin, Hong Kong, China
    2. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China
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  • Vincent Eng-Choon Ooi,

    1. Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China
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  • Mary Miu-Yee Waye

    Corresponding author
    1. Department of Biochemistry, The Croucher Laboratory for Human Genomics, The Chinese University of Hong Kong, Shatin, Hong Kong, China
    • Department of Biochemistry, The Chinese University of Hong Kong Shatin, MMW Building, Room 608, N.T., Hong Kong, China.
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Abstract

Ethanolic extract of Phyllanthus nanus (P. nanus) treatment exhibited potent antiviral activity against Hepatitis B virus (HBV). The effects of these extracts on HBV in the HBV genome integrated cell lines—Alexander cells and HepG2 2.2.15 cells were examined. Experimental results showed that the ethanolic extract of P. nanus produced suppressive effect on HBsAg secretion and HBsAg mRNA expression. The extract also inhibited HBV replication as measured by HBV DNA level in vitro. In addition, using a duck HBV (DHBV) primary culture model, the P. nanus ethanolic extract suppressed viral replication of DHBV in DHBV infected primary duck hepatocytes. The gene expression pattern in Alexander cells that had been treated with the ethanolic extract of P. nanus was also revealed by microarray techniques. The microarray results indicated that there was up-regulation of expression of several genes, including annexin A7 (Axn7). The subcellular localization of Axn7 and anti-HBV effect of Axn7 over-expression in Alexander cells were also investigated. Results showed that expression of Axn7–GFP fusion protein are localized around the secretory vesicles and could cause a decrease in HBsAg secretion in Alexander cells. Axn7 protein might play an important role in the medicinal effect of the active principle(s) of P. nanus. J. Cell. Biochem. 97: 795–812, 2006. © 2005 Wiley-Liss, Inc.

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