Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue

Authors

  • Reza Izadpanah,

    1. Division of Gene Therapy, Tulane National Primate Center, Tulane University Health Sciences Center, Covington, Louisiana
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  • Cynthia Trygg,

    1. Division of Gene Therapy, Tulane National Primate Center, Tulane University Health Sciences Center, Covington, Louisiana
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  • Bindiya Patel,

    1. Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, Louisiana
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  • Christopher Kriedt,

    1. Division of Gene Therapy, Tulane National Primate Center, Tulane University Health Sciences Center, Covington, Louisiana
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  • Jason Dufour,

    1. Division of Veterinary Medicine, Tulane National Primate Center, Tulane University Health Sciences Center, Covington, Louisiana
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  • Jeffery M. Gimble,

    1. Stem Cell Laboratory, Pennington Biomedical Research Center, Baton Rouge, Louisiana
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  • Bruce A. Bunnell

    Corresponding author
    1. Division of Gene Therapy, Tulane National Primate Center, Tulane University Health Sciences Center, Covington, Louisiana
    2. Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, Louisiana
    3. Center of Gene Therapy, Tulane University Health Sciences Center, Tulane University, New Orleans, Louisiana
    • Department of Pharmacology, Division of Gene Therapy, Center for Gene Therapy, Tulane National Primate Research Center, Tulane University Health Sciences Center, 18703 Three Rivers Road, Covington, LA 70433.
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Abstract

The biologic characteristics of mesenchymal stem cells (MSCs) isolated from two distinct tissues, bone marrow and adipose tissue were evaluated in these studies. MSCs derived from human and non-human primate (rhesus monkey) tissue sources were compared. The data indicate that MSCs isolated from rhesus bone marrow (rBMSCs) and human adipose tissue (hASCs) had more similar biologic properties than MSCs of rhesus adipose tissue (rASCs) and human bone marrow MSCs (hBMSCs). Analyses of in vitro growth kinetics revealed shorter doubling time for rBMSCs and hASCs. rBMSCs and hASCs underwent significantly more population doublings than the other MSCs. MSCs from all sources showed a marked decrease in telomerase activity over extended culture; however, they maintained their mean telomere length. All of the MSCs expressed embryonic stem cell markers, Oct-4, Rex-1, and Sox-2 for at least 10 passages. Early populations of MSCs types showed similar multilineage differentiation capability. However, only the rBMSCs and hASCs retain greater differentiation efficiency at higher passages. Overall in vitro characterization of MSCs from these two species and tissue sources revealed a high level of common biologic properties. However, the results demonstrate clear biologic distinctions, as well. J. Cell. Biochem. 99: 1285–1297, 2006. © 2006 Wiley-Liss, Inc.

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