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Anti-Thy-1 antibody-induced neurite outgrowth in cultured dorsal root ganglionic neurons is mediated by the c-Src-MEK signaling pathway

  1. Shih-Hung Yang1,2,
  2. Yi-Jen Chen1,
  3. Po-Yuan Tung1,
  4. Wei-Ling Lai1,
  5. Ying Chen1,
  6. Chung-Jiuan Jeng3,*,
  7. Seu-Mei Wang1,*

Article first published online: 7 MAY 2007

DOI: 10.1002/jcb.21387

Issue

Journal of Cellular Biochemistry

Journal of Cellular Biochemistry

Volume 103, Issue 1, pages 67–77, 1 January 2008

Additional Information

How to Cite

Yang, S.-H., Chen, Y.-J., Tung, P.-Y., Lai, W.-L., Chen, Y., Jeng, C.-J. and Wang, S.-M. (2008), Anti-Thy-1 antibody-induced neurite outgrowth in cultured dorsal root ganglionic neurons is mediated by the c-Src-MEK signaling pathway. J. Cell. Biochem., 103: 67–77. doi: 10.1002/jcb.21387

Author Information

  1. 1

    Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan

  2. 2

    Department of Surgery, Division of Neurosurgery, National Taiwan University Hospital, Taipei, Taiwan

  3. 3

    Institute of Anatomy and Cell Biology, National Yang-Ming University, Taipei, Taiwan

*Institute of Anatomy and Cell Biology, National Yang-Ming University, Taipei, Taiwan.

Publication History

  1. Issue published online: 14 DEC 2007
  2. Article first published online: 7 MAY 2007
  3. Manuscript Accepted: 27 FEB 2007
  4. Manuscript Received: 13 FEB 2007

Funded by

  • The National Science Council, Republic of China. Grant Numbers: NSC 94-2320-B-002-052, NSC 95-2314-B-002-192

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Keywords:

  • DRG neuron;
  • anti-Thy-1 antibody;
  • neurite outgrowth;
  • c-Src kinase;
  • MEK;
  • CREB

Abstract

Our previous study has shown that anti-Thy-1 antibody promotes neurite outgrowth of cultured dorsal root ganglion (DRG) neurons in a protein kinase A (PKA)-dependent manner. The present study provided another intracellular signaling pathway for the neurotrophic effect of anti-Thy-1 antibody. In DMSO-treated control cells, Thy-1 was enriched in microdomain-like structures on cell membranes by immunofluorescence observation. Treatment of DRG neurons with anti-Thy-1 antibody not only stimulated neurite outgrowth, but also increased the branching complexity of the neurites in both small and large neurons. We have previously shown that anti-Thy-1 antibody causes a time-dependent activation of mitogen-activated protein kinase (MEK) and of cyclic AMP response-element binding protein (CREB). Here, anti-Thy-1 antibody elicited a transient activation of c-Src kinase, and the activation of c-Src kinase appeared occurring upstream of the activation of MEK and CREB, since pretreatment with the Src kinase inhibitor, PP2, effectively abolished the anti-Thy-1 antibody-induced neurite outgrowth and the phosphorylation of MEK and CREB. CREB phosphorylation might result in upregulation of certain neurite outgrowth-related proteins. We therefore conclude that anti-Thy-1 antibody activates the c-Src kinase-MEK-CREB cascade and overcomes the inhibitory effect of Thy-1 on neurite outgrowth in DRG neurons. J. Cell. Biochem. 103: 67–77, 2008. © 2007 Wiley-Liss, Inc.

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