Article

Proteomic analysis reveals upregulation of RKIP in S-180 implanted BALB/C mouse after treatment with ascorbic acid

  1. Seyeon Park1,*,
  2. Eun Sook Ahn1,
  3. Sukchan Lee2,
  4. Manyong Jung2,
  5. Jin Hee Park2,
  6. Sang Yeop Yi3,
  7. Chang-Hwan Yeom4,*

Article first published online: 17 FEB 2009

DOI: 10.1002/jcb.22097

Issue

Journal of Cellular Biochemistry

Journal of Cellular Biochemistry

Volume 106, Issue 6, pages 1136–1145, 15 April 2009

Additional Information

How to Cite

Park, S., Ahn, E. S., Lee, S., Jung, M., Park, J. H., Yi, S. Y. and Yeom, C.-H. (2009), Proteomic analysis reveals upregulation of RKIP in S-180 implanted BALB/C mouse after treatment with ascorbic acid. J. Cell. Biochem., 106: 1136–1145. doi: 10.1002/jcb.22097

Author Information

  1. 1

    Department of Applied Chemistry, Dongduk Women's University, Seoul, Korea

  2. 2

    Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea

  3. 3

    Department of Pathology, Kwandong University College of Medicine Myongji Hospital, Goyang, Korea

  4. 4

    Department of Palliative Medicine, Kangnam St Mary's Hospital, Catholic University, Seoul, Korea

*Department of Applied Chemistry, Dongduk Women's University, 23-1 Wolgok-dong, Sungbuk-ku, Seoul 136-714, Korea.

Publication History

  1. Issue published online: 17 MAR 2009
  2. Article first published online: 17 FEB 2009
  3. Manuscript Accepted: 14 JAN 2009
  4. Manuscript Received: 19 NOV 2008

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Keywords:

  • Raf kinase inhibitory protein;
  • ascorbic acid;
  • proteomics

Abstract

Tumor cells have an invasive and metastatic phenotype that is the main cause of death for cancer patients. Tumor establishment and penetration consists of a series of complex processes involving multiple changes in gene expression. In this study, intraperitoneal administration of a high concentration of ascorbic acid inhibited tumor establishment and increased survival of BALB/C mice implanted with S-180 sarcoma cancer cells. To identify proteins involved in the ascorbic acid-mediated inhibition of tumor progression, changes in the liver proteome associated with ascorbic acid treatment of BALB/C mice implanted with S-180 were investigated using two-dimensional gel electrophoresis and mass spectrometry. Eleven protein spots were identified whose expression was different between control and ascorbic acid treatment groups. In particular, Raf kinase inhibitory protein (RKIP) and annexin A5 expression were quantitatively up-regulated. The increase in RKIP protein level was detected in the tumor tissue and accompanied by an increase in mRNA level. Our results suggest a possibility that these proteins are related to the ascorbic acid-mediated suppression of tumor formation. J. Cell. Biochem. 106: 1136–1145, 2009. © 2009 Wiley-Liss, Inc.

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