Proteomic analysis reveals upregulation of RKIP in S-180 implanted BALB/C mouse after treatment with ascorbic acid
Article first published online: 17 FEB 2009
Copyright © 2009 Wiley-Liss, Inc.
Journal of Cellular Biochemistry
Volume 106, Issue 6, pages 1136–1145, 15 April 2009
How to Cite
Park, S., Ahn, E. S., Lee, S., Jung, M., Park, J. H., Yi, S. Y. and Yeom, C.-H. (2009), Proteomic analysis reveals upregulation of RKIP in S-180 implanted BALB/C mouse after treatment with ascorbic acid. J. Cell. Biochem., 106: 1136–1145. doi: 10.1002/jcb.22097
- Issue published online: 17 MAR 2009
- Article first published online: 17 FEB 2009
- Manuscript Accepted: 14 JAN 2009
- Manuscript Received: 19 NOV 2008
- Raf kinase inhibitory protein;
- ascorbic acid;
Tumor cells have an invasive and metastatic phenotype that is the main cause of death for cancer patients. Tumor establishment and penetration consists of a series of complex processes involving multiple changes in gene expression. In this study, intraperitoneal administration of a high concentration of ascorbic acid inhibited tumor establishment and increased survival of BALB/C mice implanted with S-180 sarcoma cancer cells. To identify proteins involved in the ascorbic acid-mediated inhibition of tumor progression, changes in the liver proteome associated with ascorbic acid treatment of BALB/C mice implanted with S-180 were investigated using two-dimensional gel electrophoresis and mass spectrometry. Eleven protein spots were identified whose expression was different between control and ascorbic acid treatment groups. In particular, Raf kinase inhibitory protein (RKIP) and annexin A5 expression were quantitatively up-regulated. The increase in RKIP protein level was detected in the tumor tissue and accompanied by an increase in mRNA level. Our results suggest a possibility that these proteins are related to the ascorbic acid-mediated suppression of tumor formation. J. Cell. Biochem. 106: 1136–1145, 2009. © 2009 Wiley-Liss, Inc.