Lay Hoon Lee and Mei Chung Moh contributed equally to this work.
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Article
The immunoglobulin-like cell adhesion molecule hepaCAM induces differentiation of human glioblastoma U373-MG cells†
Article first published online: 8 JUN 2009
DOI: 10.1002/jcb.22215
Copyright © 2009 Wiley-Liss, Inc.
Additional Information
How to Cite
Lee, L. H., Moh, M. C., Zhang, T. and Shen, S. (2009), The immunoglobulin-like cell adhesion molecule hepaCAM induces differentiation of human glioblastoma U373-MG cells. J. Cell. Biochem., 107: 1129–1138. doi: 10.1002/jcb.22215
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Publication History
- Issue published online: 23 JUL 2009
- Article first published online: 8 JUN 2009
- Manuscript Accepted: 22 APR 2009
- Manuscript Received: 10 FEB 2009
Funded by
- National University of Singapore Academic Research Fund Grant. Grant Numbers: R-185-000-112-112, R-185-000-169-112
- Singapore Millennium Foundation Postdoctoral Fellowship Grant
- Abstract
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- References
- Cited By
Keywords:
- hepaCAM;
- immunoglobulin-like cell adhesion molecule;
- differentiation;
- glioblastoma U373-MG;
- GlialCAM
Abstract
Subsequent to our identification of a novel immunoglobulin-like cell adhesion molecule hepaCAM, we showed that hepaCAM is frequently lost in diverse human cancers and is capable of modulating cell motility and growth when re-expressed. Very recently, a molecule identical to hepaCAM (designated as GlialCAM) was found highly expressed in glial cells of the brain. Here, we demonstrate that hepaCAM is capable of inducing differentiation of the human glioblastoma U373-MG cells. Expression of hepaCAM resulted in a significant increase in the astrocyte differentiation marker glial fibrillary acid protein (GFAP), indicating that hepaCAM promotes glioblastoma cells to undergo differentiation. To determine the relationship between hepaCAM expression level and cell differentiation, we established two U373-MG cell lines expressing hepaCAM at different levels. The results revealed that high-level hepaCAM triggered a clear increase in GFAP expression as well as morphological changes characteristic of glioblastoma cell differentiation. Furthermore, high expression of hepaCAM significantly accelerated cell adhesion but inhibited cell proliferation and migration. Concomitantly, deregulation of cell cycle regulatory proteins was detected. Expectedly, the differentiation was noticeably less apparent in cells expressing low-level hepaCAM. Taken together, our findings suggest that hepaCAM induces differentiation of the glioblastoma U373-MG cells. The degree of cell differentiation is dependent on the expression level of hepaCAM. J. Cell. Biochem. 107: 1129–1138, 2009. © 2009 Wiley-Liss, Inc.