Paranthaman Sripriya and Vedantam Lakshmi Vasudev contributed equally to this work.
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Article
Involvement of mitochondria and metacaspase elevation in harpinPss-induced cell death of Saccharomyces cerevisiae†
Article first published online: 8 JUN 2009
DOI: 10.1002/jcb.22217
Copyright © 2009 Wiley-Liss, Inc.
Additional Information
How to Cite
Sripriya, P., Vedantam, L. V. and Podile, A. R. (2009), Involvement of mitochondria and metacaspase elevation in harpinPss-induced cell death of Saccharomyces cerevisiae. J. Cell. Biochem., 107: 1150–1159. doi: 10.1002/jcb.22217
- †
Publication History
- Issue published online: 23 JUL 2009
- Article first published online: 8 JUN 2009
- Manuscript Accepted: 22 APR 2009
- Manuscript Received: 24 DEC 2008
Funded by
- Department of Science and technology, Government of India. Grant Number: SP/SO/D-26/01
- Abstract
- Article
- References
- Cited By
Keywords:
- harpin;
- Saccharomyces cerevisiae;
- GAL1;
- plant hypersensitive response;
- mitochondria;
- reactive oxygen species;
- metacaspase
Abstract
Expression of a proteinaceous elicitor harpinPss, encoded by hrpZ of Pseudomonas syringae pv. syringae 61, under GAL1 promoter in Saccharomyces cerevisiae Y187 resulted in galactose-inducible yeast cell death (YCD). Extracellular treatment of harpin did not affect the growth of yeast. The observed YCD was independent of the stage of cell cycle. “Petite” mutant of S. cerevisiae Y187 pYEUT-hrpZ was insensitive to cell death indicating the involvement of mitochondria in this YCD. Loss in mitochondrial potential, but no leakage of Cytochrome c from mitochondria into the cytosol, were notable features in harpinPss-induced YCD. Cyclosporin A had no effect on hrpZ expressing yeast cells, further confirmed that there was no release of Cytochrome c. Elevation of caspase activity has been reported for the first time in this form of cell death induced by harpin expression. Release of reactive oxygen species and clear loss of membrane integrity were evident with the absence of nuclear fragmentation and chromosomal condensation, while annexin V and propidium iodide staining showed features typical of necrosis. J. Cell. Biochem. 107: 1150–1159, 2009. © 2009 Wiley-Liss, Inc.