Changes in expression of cell-cycle-related genes in PC-3 prostate cancer cells caused by ovine uterine serpin
Article first published online: 15 JUN 2009
Copyright © 2009 Wiley-Liss, Inc.
Journal of Cellular Biochemistry
Volume 107, Issue 6, pages 1182–1188, 15 August 2009
How to Cite
Padua, M. B. and Hansen, P. J. (2009), Changes in expression of cell-cycle-related genes in PC-3 prostate cancer cells caused by ovine uterine serpin. J. Cell. Biochem., 107: 1182–1188. doi: 10.1002/jcb.22222
- Issue published online: 23 JUL 2009
- Article first published online: 15 JUN 2009
- Manuscript Accepted: 30 APR 2009
- Manuscript Received: 20 MAR 2009
- uterine serpin;
- cell-cycle arrest;
- PC-3 prostate cancer cells;
The hormonal-regulated serpin, ovine uterine serpin (OvUS), also called uterine milk protein (UTMP), inhibits proliferation of lymphocytes and prostate cancer (PC-3) cells by blocking cell-cycle progression. The present aim was to identify cell-cycle-related genes regulated by OvUS in PC-3 cells using the quantitative human cell-cycle RT2 Profiler™ PCR array. Cells were cultured ±200 µg/ml recombinant OvUS (rOvUS) for 12 and 24 h. At 12 h, rOvUS increased expression of three genes related to cell-cycle checkpoints and arrest (CDKN1A, CDKN2B, and CCNG2). Also, 14 genes were down-regulated including genes involved in progression through S (MCM3, MCM5, PCNA), M (CDC2, CKS2, CCNH, BIRC5, MAD2L1, MAD2L2), G1 (CDK4, CUL1, CDKN3) and DNA damage checkpoint and repair genes RAD1 and RBPP8. At 24 h, rOvUS decreased expression of 16 genes related to regulation and progression through M (BIRC5, CCNB1, CKS2, CDK5RAP1, CDC20, E2F4, MAD2L2) and G1 (CDK4, CDKN3, TFDP2), DNA damage checkpoints and repair (RAD17, BRCA1, BCCIP, KPNA2, RAD1). Also, rOvUS down-regulated the cell proliferation marker gene MKI67, which is absent in cells at G0. Results showed that OvUS blocks cell-cycle progression through upregulation of cell-cycle checkpoint and arrest genes and down-regulation of genes involved in cell-cycle progression. J. Cell. Biochem. 107: 1182–1188, 2009. © 2009 Wiley-Liss, Inc.