Article

Effects of fibre type and diffusion distance on mouse skeletal muscle glycogen content in vitro

  1. Peter Sogaard1,2,
  2. Ferenc Szekeres1,
  3. Maria Holmström1,
  4. Dennis Larsson2,
  5. Mikael Harlén3,
  6. Pablo Garcia-Roves1,4,
  7. Alexander V. Chibalin1,*

Article first published online: 8 JUN 2009

DOI: 10.1002/jcb.22223

Issue

Journal of Cellular Biochemistry

Journal of Cellular Biochemistry

Volume 107, Issue 6, pages 1189–1197, 15 August 2009

Additional Information

How to Cite

Sogaard, P., Szekeres, F., Holmström, M., Larsson, D., Harlén, M., Garcia-Roves, P. and Chibalin, A. V. (2009), Effects of fibre type and diffusion distance on mouse skeletal muscle glycogen content in vitro. J. Cell. Biochem., 107: 1189–1197. doi: 10.1002/jcb.22223

Author Information

  1. 1

    Department of Molecular Medicine and Surgery, Section of Integrative Physiology, Karolinska Institutet, Stockholm, Sweden

  2. 2

    Systems Biology Research Centre, Department of Biomedicine, University of Skövde, P.O. Box 408, 541 28 Skövde, Sweden

  3. 3

    Systems Biology Research Centre, Department of Cell and Molecular Biology, School of Life Sciences, University of Skövde, P.O. Box 408, 541 28 Skövde, Sweden

  4. 4

    Section of Integrative Physiology, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden

*Section of Integrative Physiology, Department of Molecular Medicine and Surgery, von Eulers vag 4, 4 tr, Karolinska Institutet, 171 77 Stockholm, Sweden.

Publication History

  1. Issue published online: 23 JUL 2009
  2. Article first published online: 8 JUN 2009
  3. Manuscript Accepted: 30 APR 2009
  4. Manuscript Received: 3 MAR 2009

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Keywords:

  • immunohistochemistry;
  • muscle glycogen;
  • fibre type;
  • insulin action;
  • anoxia

Abstract

In vitro incubation of isolated rodent skeletal muscle is a widely used procedure in metabolic research. One concern with this method is the development of an anoxic state during the incubation period that can cause muscle glycogen depletion. Our aim was to investigate whether in vitro incubation conditions influence glycogen concentration in glycolytic extensor digitorum longus (EDL) and oxidative soleus mouse muscle. Quantitative immunohistochemistry was applied to assess glycogen content in incubated skeletal muscle. Glycogen concentration was depleted, independent of insulin-stimulation in the incubated skeletal muscle. The extent of glycogen depletion was correlated with the oxidative fibre distribution and with the induction of hypoxia-induced-factor-1-alpha. Insulin exposure partially prevented glycogen depletion in soleus, but not in EDL muscle, providing evidence that glucose diffusion is not a limiting step to maintain glycogen content. Our results provide evidence to suggest that the anoxic milieu and the intrinsic characteristics of the skeletal muscle fibre type play a major role in inducing glycogen depletion in during in vitro incubations. J. Cell. Biochem. 107: 1189–1197, 2009. © 2009 Wiley-Liss, Inc.

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