Article

Collection and culture of alveolar bone marrow multipotent mesenchymal stromal cells from older individuals

  1. Juan Han1,2,
  2. Hironori Okada3,
  3. Hideki Takai2,4,
  4. Youhei Nakayama2,4,
  5. Takahide Maeda1,2,
  6. Yorimasa Ogata2,4,*

Article first published online: 8 JUN 2009

DOI: 10.1002/jcb.22224

Issue

Journal of Cellular Biochemistry

Journal of Cellular Biochemistry

Volume 107, Issue 6, pages 1198–1204, 15 August 2009

Additional Information

How to Cite

Han, J., Okada, H., Takai, H., Nakayama, Y., Maeda, T. and Ogata, Y. (2009), Collection and culture of alveolar bone marrow multipotent mesenchymal stromal cells from older individuals. J. Cell. Biochem., 107: 1198–1204. doi: 10.1002/jcb.22224

Author Information

  1. 1

    Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan

  2. 2

    Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan

  3. 3

    Two Cells Co., Ltd., Hiroshima, Hiroshima, Japan

  4. 4

    Department of Periodontology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan

*Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan.

Publication History

  1. Issue published online: 23 JUL 2009
  2. Article first published online: 8 JUN 2009
  3. Manuscript Accepted: 30 APR 2009
  4. Manuscript Received: 11 OCT 2008

Funded by

  • Japan Society for the Promotion of Science (JSPS). Grant Number: 18390563
  • Supporting Project for Strategic Research by the Ministry of Education, Culture, Sports, Science and Technology. Grant Number: 2008-2012

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Keywords:

  • regenerative medicine;
  • oral;
  • alveolar;
  • bone marrow;
  • stroma;
  • older individuals

Abstract

In this work, we examined the culture condition of alveolar bone marrow multipotent mesenchymal stromal cells (ABMMSCs), aiming to apply regenerative therapy to older periodontitis patients. To better understand the character of cultured cells from alveolar bone marrow, the expression profiles of well-known genes and their responses to the induction of osteogenic, chondrogenic, or adipogenic differentiation were examined. Using αMEM-based culture, ABMMSCs could be obtained from older individuals than in previous reports. Interestingly, ABMMSCs expressing Klf4 were able to differentiate into osteoblasts. The prediction of differentiation potential by Klf4 could be a useful guide for further improvement of the culture conditions required to culture ABMMSCs derived from older individuals. J. Cell. Biochem. 107: 1198–1204, 2009. © 2009 Wiley-Liss, Inc.

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