Article

The activation of matrix metalloproteinase-2 induced by protein kinase C alpha in decidualization†

  1. Jen-Hsiang Tsai1,
  2. Jin-Ming Hwang2,
  3. Tsung-Ho Ying3,
  4. Jyh-Cherng Shyu4,
  5. Chin-Chiu Tsai4,
  6. Yih-Shou Hsieh5,
  7. Yi-Wei Wang5,
  8. Jer-Yuh Liu5,*,
  9. Shao-Hsuan Kao5,*

Article first published online: 19 AUG 2009

DOI: 10.1002/jcb.22285

Issue

Journal of Cellular Biochemistry

Journal of Cellular Biochemistry

Volume 108, Issue 3, pages 547–554, 15 October 2009

Additional Information

How to Cite

Tsai, J.-H., Hwang, J.-M., Ying, T.-H., Shyu, J.-C., Tsai, C.-C., Hsieh, Y.-S., Wang, Y.-W., Liu, J.-Y. and Kao, S.-H. (2009), The activation of matrix metalloproteinase-2 induced by protein kinase C alpha in decidualization. Journal of Cellular Biochemistry, 108: 547–554. doi: 10.1002/jcb.22285

Author Information

  1. 1

    Department of Physical Therapy, School of Medical and Health Sciences, Fooyin University, Kaohsiung 83102, Taiwan

  2. 2

    Department of Applied Chemistry, School of Medicine, College of Medicine, Chung Shan Medical University, Taichung 40203, Taiwan

  3. 3

    Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Chung Shan Medical University, Taichung 40203, Taiwan

  4. 4

    Department of Physiology, School of Medicine, College of Medicine, Chung Shan Medical University, Taichung 40203, Taiwan

  5. 5

    Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, Taichung 40203, Taiwan

*Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Chien Kuo N. Road, Taichung 40203, Taiwan.

  1. †

    Shao-Hsuan Kao and Jer-Yuh Liu contributed equally to this work.

Publication History

  1. Issue published online: 22 SEP 2009
  2. Article first published online: 19 AUG 2009
  3. Manuscript Accepted: 22 JUN 2009
  4. Manuscript Received: 20 MAY 2009

Funded by

  • The National Science Council (NSC), Republic of China. Grant Number: 95-2320-B-040-043
  • Chung Shan Medical University (CSMU), Republic of China. Grant Numbers: 96-OM-B-040, 87-OM-B-002, 88-OM-B-008

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Keywords:

  • matrix metalloproteinase-2;
  • protein kinase C;
  • decidualization

Abstract

This study investigated the protein kinase C (PKC) and matrix metalloproteinase-2 (MMP-2) in the development of deciduomata in pseudo-pregnant and pregnant rats. The results showed that the expression of MMP-2 was significantly increased from day 2 to day 5 in pseudo-pregnancy and from day 7 to day 9 in pregnancy. To further investigate the correlation between MMP-2 and protein kinase Cα (PKCα), the expression of MMP-2 in the 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated organotypic culture of decidual tissue was determined. The results showed that the active form of MMP-2 was significantly increased in the TPA-treated cultures. Moreover, this response was inhibited by the PKC inhibitor H7, the PKCα specific inhibitor Gö-6976 and the translation inhibitor cycloheximide, but not by the transcription inhibitor actinomycin D or the replication inhibitor mitomycin C. In addition, TPA also reversed the MMP-2 expression after by progesterone pretreatment in the primary decidual cells. These findings indicate that PKCα may play an important role in the regulation of the MMP-2 expression during decidualization. J. Cell. Biochem. 108: 547–554, 2009. © 2009 Wiley-Liss, Inc.

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