Article

Swiprosin-1 is expressed in mast cells and up-regulated through the protein kinase CβI/η pathway

  1. Ramesh P. Thylur1,
  2. Young-Dae Kim1,
  3. Min-Sung Kwon1,
  4. Hyun-Mee Oh1,
  5. Ho-Keun Kwon1,
  6. Sang-Hyun Kim2,
  7. Sin-Hyeog Im1,
  8. Jang-Soo Chun1,
  9. Zee-Yong Park1,
  10. Chang-Duk Jun1,*

Article first published online: 19 AUG 2009

DOI: 10.1002/jcb.22307

Issue

Journal of Cellular Biochemistry

Journal of Cellular Biochemistry

Volume 108, Issue 3, pages 705–715, 15 October 2009

Additional Information

How to Cite

Thylur, R. P., Kim, Y.-D., Kwon, M.-S., Oh, H.-M., Kwon, H.-K., Kim, S.-H., Im, S.-H., Chun, J.-S., Park, Z.-Y. and Jun, C.-D. (2009), Swiprosin-1 is expressed in mast cells and up-regulated through the protein kinase CβI/η pathway. Journal of Cellular Biochemistry, 108: 705–715. doi: 10.1002/jcb.22307

Author Information

  1. 1

    Department of Life Science, Cell Dynamics Research Center, BioImaging Research Center, and Research Center for Biomolecular Nanotechnology, GIST, Gwangju 500-712, South Korea

  2. 2

    Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, South Korea

*Department of Life Science, GIST, Gwangju 500-712, South Korea.

Publication History

  1. Issue published online: 22 SEP 2009
  2. Article first published online: 19 AUG 2009
  3. Manuscript Accepted: 14 JUL 2009
  4. Manuscript Received: 15 JUN 2009

Funded by

  • MEST/KOSEF. Grant Numbers: R01-2008-000-20989-0, R11-2007-007-01002-0
  • Korea Research Foundation. Grant Number: 2008-C00265

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Keywords:

  • mast cells;
  • swiprosin-1;
  • protein kinase C;
  • calcium

Abstract

Swiprosin-1 exhibits the highest expression in CD8+ T cells and immature B cells and has been thought to play a role in lymphocyte physiology. Here we report that swiprosin-1 is also expressed in mast cells and up-regulated in both in vitro cultured mast cells by phorbol ester and in vivo model tissues of passive cutaneous anaphylaxis and atopic dermatitis. Targeted inhibition of the specific protein kinase C (PKC) isotypes by siRNA revealed that PKC-βI/η are involved in the expression of swiprosin-1 in the human mast cell line HMC-1. In contrast, down-regulation of swiprosin-1 by A23187 or ionomycin suggests that calcium-signaling plays a negative role. The ectopic expression of swiprosin-1 augmented PMA/A23187-induced NF-κB promoter activity, and resulted in increased expression of cytokines. Moreover, knock-down of swiprosin-1 attenuated PMA/A23187-induced cytokine expression. Collectively, these results suggest that swiprosin-1 is a PKC-βI/η-inducible gene and it modulates mast cell activation through NF-κB-dependent pathway. J. Cell. Biochem. 108: 705–715, 2009. © 2009 Wiley-Liss, Inc.

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