Get access
Advertisement

Proteomic analysis of differentially expressed proteins in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory–secretory products

Authors

  • Jhang Ho Pak,

    Corresponding author
    1. Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, South Korea
    • Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, 388-1 Pungnap-2 dong, Songpa-gu, Seoul 138-736, South Korea.
    Search for more papers by this author
  • Ju Hyun Moon,

    1. Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, South Korea
    Search for more papers by this author
  • Seung-Jun Hwang,

    1. Department of Anatomy and Cell Biology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, South Korea
    Search for more papers by this author
  • Shin-Hyeong Cho,

    1. Division of Malaria and Parasitic Diseases, National Institute of Health, Seoul 122-701, South Korea
    Search for more papers by this author
  • Sang-Beom Seo,

    1. Deptartment of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, South Korea
    Search for more papers by this author
  • Tong-Soo Kim

    Corresponding author
    1. Deptartment of Parasitology, Inha University School of Medicine, Inchon 400-103, South Korea
    • Department of Parasitology, Inha University School of Medicine, Shinheung-dong, Choong-gu, Inchon 400-712, South Korea.
    Search for more papers by this author

Abstract

Severe Clonorchis sinensis infection is a significant risk factor for malignant changes in bile ducts and surrounding liver tissues occurring as a result of direct contact with C. sinensis worms and their excretory–secretory products (ESP). However, the intrinsic molecular mechanisms involved in these processes remain obscure. To determine the effects of C. sinensis infection on protein expression in host bile duct epithelium, we examined proteomic profile changes in the human cholangiocarcinoma cell line (HuCCT1) treated with ESP at 24 h. Using a combination of 2-DE, quantitative image and MALDI-TOF MS analysis, we identified 83 proteins that were translationally modulated in response to ESP, among which 49 were up-regulated and 34 down-regulated. These proteins were classified under various biological categories, including metabolism, cell structure and architecture, proteolysis, protein modification, transport, signal transduction, and reactive oxygen species (ROS) detoxification. In particular, ESP induced the expression of redox-regulating proteins, including peroxiredoxins (Prdx 2, 3, and 6) and thioredoxin 1 (Trx 1), possibly via intracellular ROS generation. Application of the proteomic approach to identify ESP response proteins should be a prerequisite before further investigation to clarify the molecular pathways and mechanisms involved in C. sinensis infection of host cells. J. Cell. Biochem. 108: 1376–1388, 2009. © 2009 Wiley-Liss, Inc.

Get access to the full text of this article

Ancillary