Journal of Cellular Biochemistry

Effect of IL-1β, PGE2, and TGF-β1 on the expression of OPG and RANKL in normal and osteoporotic primary human osteoblasts

Authors

  • Susana Jurado,

    Corresponding author
    1. Internal Medicine, URFOA, IMIM, RETICEF, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Spain
    • Dr Aiguader 88, 08003 Barcelona, Spain.
    Search for more papers by this author
  • Natalia Garcia-Giralt,

    1. Internal Medicine, URFOA, IMIM, RETICEF, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Spain
    Search for more papers by this author
  • Adolfo Díez-Pérez,

    1. Internal Medicine, URFOA, IMIM, RETICEF, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Spain
    Search for more papers by this author
  • Pedro Esbrit,

    1. Laboratorio de Metabolismo Mineral y Óseo, Fundación Jiménez Díaz (Capio Group), Madrid, Spain
    Search for more papers by this author
  • Guy Yoskovitz,

    1. Internal Medicine, URFOA, IMIM, RETICEF, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Spain
    Search for more papers by this author
  • Lídia Agueda,

    1. Faculty of Biology, Department of Genetics, University of Barcelona, Barcelona, Spain
    2. Institut de Biomedicina de la Universitat de Barcelona (IBUB), Barcelona, Spain
    3. CIBER de Enfermedades Raras (CIBERER), Barcelona, Spain
    Search for more papers by this author
  • Roser Urreizti,

    1. Faculty of Biology, Department of Genetics, University of Barcelona, Barcelona, Spain
    2. Institut de Biomedicina de la Universitat de Barcelona (IBUB), Barcelona, Spain
    3. CIBER de Enfermedades Raras (CIBERER), Barcelona, Spain
    Search for more papers by this author
  • Lluís Pérez-Edo,

    1. Department of Rheumatology, URFOA, IMIM, RETICEF, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Spain
    Search for more papers by this author
  • Guillem Saló,

    1. Department of Traumatology and Orthopaedic Surgery, URFOA, IMIM, RETICEF, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Spain
    Search for more papers by this author
  • Leonardo Mellibovsky,

    1. Internal Medicine, URFOA, IMIM, RETICEF, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Spain
    Search for more papers by this author
  • Susana Balcells,

    1. Faculty of Biology, Department of Genetics, University of Barcelona, Barcelona, Spain
    2. Institut de Biomedicina de la Universitat de Barcelona (IBUB), Barcelona, Spain
    3. CIBER de Enfermedades Raras (CIBERER), Barcelona, Spain
    Search for more papers by this author
  • Daniel Grinberg,

    1. Faculty of Biology, Department of Genetics, University of Barcelona, Barcelona, Spain
    2. Institut de Biomedicina de la Universitat de Barcelona (IBUB), Barcelona, Spain
    3. CIBER de Enfermedades Raras (CIBERER), Barcelona, Spain
    Search for more papers by this author
  • Xavier Nogués

    1. Internal Medicine, URFOA, IMIM, RETICEF, Hospital del Mar, Autonomous University of Barcelona, Barcelona, Spain
    Search for more papers by this author

Abstract

The RANKL/RANK/OPG pathway is essential for bone remodeling regulation. Many hormones and cytokines are involved in regulating gene expression in most of the pathway components. Moreover, any deregulation of this pathway can alter bone metabolism, resulting in loss or gain of bone mass. Whether osteoblasts from osteoporotic and nonosteoporotic patients respond differently to cytokines is unknown. The aim of this study was to compare the effect of interleukin (IL)-1β, proftaglandin E2 (PGE2), and transforming growth factor-β1 (TGF-β1) treatments on OPG and RANKL gene expression in normal (n = 11) and osteoporotic (n = 8) primary osteoblasts. OPG and RANKL mRNA levels of primary human osteoblastic (hOB) cell cultures were assessed by real-time PCR. In all cultures, OPG mRNA increased significantly in response to IL-1β treatment and decreased in response to TGF-β1 whereas PGE2 treatment had no effect. RANKL mRNA levels were significantly increased by all treatments. Differences in OPG and RANKL responses were observed between osteoporotic and nonosteoporotic hOB: in osteoporotic hOB, the OPG response to IL-1β treatment was up to three times lower (P = 0.009), whereas that of RANKL response to TGF-β1 was five times higher (P = 0.002) after 8 h of treatment, as compared with those in nonosteoporotic hOBs. In conclusion, osteoporotic hOB cells showed an anomalous response under cytokine stimulation, consistent with an enhanced osteoclastogenesis resulting in high levels of bone resorption. J. Cell. Biochem. 110: 304–310, 2010. © 2010 Wiley-Liss, Inc.

Ancillary