Brahmananda R. Chitteti and Ying-Hua Cheng contributed equally to this work.
Osteoblast lineage cells expressing high levels of Runx2 enhance hematopoietic progenitor cell proliferation and function†
Article first published online: 12 MAY 2010
Copyright © 2010 Wiley-Liss, Inc.
Journal of Cellular Biochemistry
Volume 111, Issue 2, pages 284–294, 1 October 2010
How to Cite
Chitteti, B. R., Cheng, Y.-H., Streicher, D. A., Rodriguez-Rodriguez, S., Carlesso, N., Srour, E. F. and Kacena, M. A. (2010), Osteoblast lineage cells expressing high levels of Runx2 enhance hematopoietic progenitor cell proliferation and function. J. Cell. Biochem., 111: 284–294. doi: 10.1002/jcb.22694
- Issue published online: 12 MAY 2010
- Article first published online: 12 MAY 2010
- Accepted manuscript online: 19 AUG 2010 12:00AM EST
- Manuscript Accepted: 29 APR 2010
- Manuscript Received: 3 MAR 2010
- NIH. Grant Number: R01 HL55716
- NCI. Grant Number: P30 CA082709
- hematopoietic stem cell niche;
- alkaline phosphatase;
- hematopoietic progenitor cells
Although osteoblasts (OB) play a key role in the hematopoietic stem cell (HSC) niche, little is known as to which specific OB lineage cells are critical for the enhancement of stem and progenitor cell function. Unlike hematopoietic cells, OB cell surface phenotypic definitions are not well developed. Therefore, to determine which OB lineage cells are most important for hematopoietic progenitor cell (HPC) function, we characterized OB differentiation by gene expression and OB function, and determined whether associations existed between OB and HPC properties. OB were harvested from murine calvariae, used immediately (fresh OB) or cultured for 1, 2, or 3 weeks prior to their co-culture with Lin−Sca1+c-kit+ (LSK) cells for 1 week. OB gene expression, alkaline phosphatase activity, calcium deposition, hematopoietic cell number fold increase, CFU fold increase, and fold increase of Lin−Sca1+ cells were determined. As expected, HPC properties were enhanced when LSK cells were cultured with OB compared to being cultured alone. Initial alkaline phosphatase and calcium deposition levels were significantly and inversely associated with an increase in the number of LSK progeny. Final calcium deposition levels and OB culture duration were inversely associated with all HPC parameters, while Runx2 levels were positively associated with all HPC properties. Since calcium deposition is associated with OB maturation and high levels of Runx2 are associated with less mature OB lineage cells, these results suggest that less mature OB better promote HPC proliferation and function than do more mature OB. J. Cell. Biochem. 111: 284–294, 2010. © 2010 Wiley-Liss, Inc.