IL-8 increases integrin expression and cell motility in human chondrosarcoma cells
Article first published online: 18 AUG 2011
Copyright © 2011 Wiley-Liss, Inc.
Journal of Cellular Biochemistry
Volume 112, Issue 9, pages 2549–2557, September 2011
How to Cite
Lee, C.-Y., Huang, C.-Y., Chen, M.-Y., Lin, C.-Y., Hsu, H.-C. and Tang, C.-H. (2011), IL-8 increases integrin expression and cell motility in human chondrosarcoma cells. J. Cell. Biochem., 112: 2549–2557. doi: 10.1002/jcb.23179
- Issue published online: 18 AUG 2011
- Article first published online: 18 AUG 2011
- Accepted manuscript online: 16 MAY 2011 10:07AM EST
- Manuscript Accepted: 4 MAY 2011
- Manuscript Received: 23 FEB 2011
- National Science Council of Taiwan. Grant Numbers: NSC99-2628-B-002-025-MY3, NSC99-2628-B-002-014-MY3
- China Medical University. Grant Number: CMU99-ASIA-10
Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. Interleukin-8 (IL-8), a chemokine with a defining CXC amino acid motif, is known to possess tumorigenic and proangiogenic properties. Over-expression of IL-8 has been detected in many human tumors. However, the effects of IL-8 in migration and integrin expression in chondrosarcoma cells are largely unknown. In this study, we found that IL-8 increased the migration and the expression of αvβ3 integrin in human chondrosarcoma cells. Activations of phosphatidylinositol 3-kinase (PI3K), Akt, and AP-1 pathways after IL-8 treatment were demonstrated, and IL-8-induced expression of integrin and migration activity was inhibited by the specific inhibitor and mutant of PI3K, Akt, and AP-1 cascades. Taken together, our results indicated that IL-8 enhances the migration of chondrosarcoma cells by increasing αvβ3 integrin expression through the PI3K/Akt/AP-1 signal transduction pathway. J. Cell. Biochem. 112: 2549–2557, 2011. © 2011 Wiley-Liss, Inc.