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Journal of Cellular Biochemistry

Effects of nonylphenol on the production of progesterone on the rats granulosa cells

Authors

  • Po-Ling Yu,

    1. Department of Surgery, Taipei City Hospital, Taipei 10431, Taiwan, Republic of China
    2. Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China
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  • Han-Wei Lin,

    1. Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China
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  • Shyi-Wu Wang,

    1. Department of Physiology and Pharmacology, College of Medicine, Chang-Gung University, Taoyuan 33333, Taiwan, Republic of China
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  • Paulus S. Wang

    Corresponding author
    1. Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China
    2. Department of Medical Research and Education, Taipei City Hospital, Taipei 10431, Taiwan, Republic of China
    3. Laboratory Animal Center, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China
    • Department of Physiology, School of Medicine, National Yang-Ming University, Shih-Pai, Taipei 11221, Taiwan, Republic of China.
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Abstract

We investigated the effects of nonylphenol (NP) on release of progesterone (PG) by granulosa cells (GCs) of rats in vitro and in vivo. First, GCs were treated with different doses of NP for 2–24 h alone or with human chorionic gonadotropin (hCG). Maximal PG secretion at 8 h noted, GCs were treated for 2 h with hCG, 8-bromo-adenosine 3′:5′-cyclic monophosphate (8-Br-cAMP), forskolin, A23187, nifedipine, and pregnelonone to evaluate the NP effects on PG steroidogenesis. Results indicated that all of chemicals except nifedipine stimulated the PG release compared to vehicle, but the stimulatory effects could not be enhanced by different doses of NP. Second, GCs were isolated to react with hCG, 8-Br-cAMP and PD98059 after the immature female rats gavaged with different doses of NP (ONP) for 7 days. PG released significantly when rats treated with oral NP 100 compared to 0 µg/kg/day. Third, GCs collected from the female offspring of mother rats which gavaged with NP 100 µg/kg/day for 21 days during pregnancy (MONP) reacted with different doses of chemicals. The results showed that PG release in the presence of chemicals was significantly higher in ONP and MONP groups; however, this stimulation was not noted by dose-dependent. The plasma concentration of PG was higher in ONP (100 µg/kg/day) and the offspring of MONP groups. The steroidogenic acute regulatory (StAR) protein expressed higher in all three groups by Western blotting. This study results indicated that low dose of NP stimulated PG release in rat GCs by activation of StAR protein. J. Cell. Biochem. 112: 2627–2636, 2011. © 2011 Wiley-Liss, Inc.

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