Differential expression of the two VEGF receptors flt and KDR in placenta and vascular endothelial cells
Article first published online: 19 FEB 2004
Copyright © 1994 Wiley-Liss, Inc.
Journal of Cellular Biochemistry
Volume 54, Issue 1, pages 56–66, January 1994
How to Cite
Barleon, B., Hauser, S., Schöllmann, C., Weindel, K., Marmé, D., Yayon, A. and Weich, H. A. (1994), Differential expression of the two VEGF receptors flt and KDR in placenta and vascular endothelial cells. J. Cell. Biochem., 54: 56–66. doi: 10.1002/jcb.240540107
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 24 AUG 1993
- Manuscript Received: 17 MAR 1993
- endothelial cells;
Vascular endothelial growth factor (VEGF) is a newly identified growth and permeability factor with a unique specificity for endothelial cells. Recently the flt-encoded tyrosine kinase was characterized as a receptor for VEGF. A novel tyrosine kinase receptor encoded by the KDR gene was also found to bind VEGF with high affinity when expressed in CMT-3 cells. Screening for flt and KDR expression in a variety of species and tissue-derived endothelial cells demonstrates that flt is predominantly expressed in human placenta and human vascular endothelial cells. Placenta growth factor (PIGF), a growth factor significantly related to VEGF, is coexpressed with flt in placenta and human vascular endothelial cells. KDR is more widely distributed and expressed in all vessel-derived endothelial cells. These data demonstrate that cultured human endothelial cells isolated from different tissues express both VEGF receptors in relative high levels and, additionally, that all investigated nonhuman endothelial cells in culture are also positive for KDR gene expression.