The authors declare that they have no conflict of interest.
Noggin suppression decreases BMP-2-induced osteogenesis of human bone marrow-derived mesenchymal stem cells In Vitro†
Article first published online: 9 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 113, Issue 12, pages 3672–3680, December 2012
How to Cite
Chen, C., Uludağ, H., Wang, Z. and Jiang, H. (2012), Noggin suppression decreases BMP-2-induced osteogenesis of human bone marrow-derived mesenchymal stem cells In Vitro. J. Cell. Biochem., 113: 3672–3680. doi: 10.1002/jcb.24240
- Issue published online: 9 OCT 2012
- Article first published online: 9 OCT 2012
- Accepted manuscript online: 27 JUN 2012 06:52AM EST
- Manuscript Accepted: 20 JUN 2012
- Manuscript Received: 27 OCT 2011
- Edmonton Orthopaedic Research Committee. Grant Number: G522000061
Additional Supporting Information may be found in the online version of this article.
|jcb_24240_sm_SupplFig1.tif||1421K||Supplementary Figure 1. Assessment of transfection efficiency in human MSCs at 24 h after siRNA transfection. Percentage (A) and mean fluorescence (B) of fluorescent dye-labelled cells (siRNA-positive cells) under different transfection conditions.|
|jcb_24240_sm_SupplFig2.tif||248K||Supplementary Figure 2. Noggin mRNA expression in cell cultures on day 14 of osteoinduction by osteogenic medium containing 0.1 µg/mL BMP-2. * * denotes p<0.01 versus both NT and Ctrl siRNA groups.|
|jcb_24240_sm_SupplTab1.doc||28K||Supplementary Table 1. Patient information.|
|jcb_24240_sm_SupplTab2.doc||31K||Supplementary Table 2. PCR primers used in this study.|
|jcb_24240_sm_SupplTab3.doc||32K||Supplementary Table 3. Combination of reagents used for transfection optimization.|
|jcb_24240_sm_SupplTab4.doc||35K||Supplementary Table 4. Data for noggin siRNAs.|
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