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Quantitative proteome analysis of ovarian cancer tissues using a iTRAQ approach

Authors

  • Li-Na Wang,

    1. Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
    2. Department of Immunology, Nankai University School of Medicine, Tianjin 300071, China
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  • Shi-Wen Tong,

    1. Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
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  • Huai-Dong Hu,

    1. Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
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  • Feng Ye,

    1. Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
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  • Sang-Lin Li,

    1. Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
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  • Hong Ren,

    1. Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
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  • Da-Zhi Zhang,

    1. Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
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  • Rong Xiang,

    Corresponding author
    1. Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
    2. Department of Immunology, Nankai University School of Medicine, Tianjin 300071, China
    • Department of Immunology, Nankai University School of Medicine, Tianjin 300071, China.
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  • Yi-Xuan Yang

    Corresponding author
    1. Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
    • Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China
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  • Li-Na Wang, Shi-Wen Tong, and Huai-Dong Hu contributed equally to this work.

Abstract

Quantitative proteomics can be used as a screening tool for identification of differentially expressed proteins as potential biomarkers for cancers. Here, we comparatively analyzed the proteome profiles of ovarian cancer tissues and normal ovarian epithelial tissues. Using the high-throughput proteomic technology of isobaric tags for relative and absolute quantitation (iTRAQ)-coupled with two-dimensional-liquid chromatography-tandem mass spectrometry, 1,259 unique proteins were identified. Of those, 205 were potentially differentially expressed between ovarian cancer and normal ovarian tissues. Several of the potentially differentially expressed proteins were validated by Western blotting and real-time quantitative RT-PCR analyses. Furthermore, up-regulation of KRT8, PPA1, IDH2, and S100A11 were validated in ovarian tissue microarrays by immunohistochemistry. Silencing of S100A11 expression suppressed the migration and invasion properties of ovarian cancer cells in vitro. Our study represents the successful application of iTRAQ technology to an investigation of ovarian cancer. Many of the potentially differentially expressed proteins identified had not been linked to ovarian cancer before, and provide valuable novel insights into the underlying mechanisms of carcinogenesis in human ovarian cancer. J. Cell. Biochem. 113: 3762–3772, 2012. © 2012 Wiley Periodicals, Inc.

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