SAP30L (Sin3A-associated protein 30-like) is involved in regulation of cardiac development and hematopoiesis in zebrafish embryos

Authors

  • Kaisa J. Teittinen,

    Corresponding author
    1. Paediatric Research Centre, University of Tampere School of Medicine and Tampere University Hospital, 33520 Tampere, Finland
    • Paediatric Research Centre, University of Tampere School of Medicine, FIN-33520 Tampere, Finland
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  • Toni Grönroos,

    1. Paediatric Research Centre, University of Tampere School of Medicine and Tampere University Hospital, 33520 Tampere, Finland
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  • Mataleena Parikka,

    1. Institute of Biomedical Technology, BioMediTech, University of Tampere, 33520 Tampere, Finland
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  • Sini Junttila,

    1. Turku Centre for Biotechnology and the Finnish Microarray and Sequencing Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland
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  • Annemari Uusimäki,

    1. Paediatric Research Centre, University of Tampere School of Medicine and Tampere University Hospital, 33520 Tampere, Finland
    2. Institute of Biomedical Technology, BioMediTech, University of Tampere, 33520 Tampere, Finland
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  • Asta Laiho,

    1. Turku Centre for Biotechnology and the Finnish Microarray and Sequencing Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland
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  • Hanna Korkeamäki,

    1. Paediatric Research Centre, University of Tampere School of Medicine and Tampere University Hospital, 33520 Tampere, Finland
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  • Kalle Kurppa,

    1. Paediatric Research Centre, University of Tampere School of Medicine and Tampere University Hospital, 33520 Tampere, Finland
    2. Department of Paediatrics, Tampere University Hospital, 33521 Tampere, Finland
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  • Hannu Turpeinen,

    1. Institute of Biomedical Technology, BioMediTech, University of Tampere, 33520 Tampere, Finland
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  • Marko Pesu,

    1. Institute of Biomedical Technology, BioMediTech, University of Tampere, 33520 Tampere, Finland
    2. Fimlab Laboratories, Tampere University Hospital, 33521 Tampere, Finland
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  • Attila Gyenesei,

    1. Turku Centre for Biotechnology and the Finnish Microarray and Sequencing Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland
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  • Mika Rämet,

    1. Institute of Biomedical Technology, BioMediTech, University of Tampere, 33520 Tampere, Finland
    2. Department of Paediatrics, Tampere University Hospital, 33521 Tampere, Finland
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  • Olli Lohi

    1. Paediatric Research Centre, University of Tampere School of Medicine and Tampere University Hospital, 33520 Tampere, Finland
    2. Department of Paediatrics, Tampere University Hospital, 33521 Tampere, Finland
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  • The authors have no conflict of interest to declare.

Abstract

The Sin3A-associated proteins SAP30 and SAP30L share 70% sequence identity and are part of the multiprotein Sin3A corepressor complex. They participate in gene repression events by linking members of the complex and stabilizing interactions among the protein members as well as between proteins and DNA. While most organisms have both SAP30 and SAP30L, the zebrafish is exceptional because it only has SAP30L. Here we demonstrate that SAP30L is expressed ubiquitously in embryonic and adult zebrafish tissues. Knockdown of SAP30L using morpholino-mediated technology resulted in a morphant phenotype manifesting as cardiac insufficiency and defective hemoglobinization of red blood cells. A microarray analysis of gene expression in SAP30L morphant embryos revealed changes in the expression of genes involved in regulation of transcription, TGF-beta signaling, Wnt-family transcription factors, and nuclear genes encoding mitochondrial proteins. The expression of the heart-specific nkx2.5 gene was markedly down-regulated in SAP30L morphants, and the cardiac phenotype could be partially rescued by nkx2.5 mRNA. In addition, changes were detected in the expression of genes known to be important in hemoglobin synthesis and erythropoiesis. Our results demonstrate that SAP30L regulates several transcriptional pathways in zebrafish embryos and is involved in the development of cardiac and hematopoietic systems. J. Cell. Biochem. 113: 3843–3852, 2012. © 2012 Wiley Periodicals, Inc.

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