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Journal of Cellular Biochemistry

Characterization of human NLZ1/ZNF703 identifies conserved domains essential for proper subcellular localization and transcriptional repression

Authors

  • Isabel Pereira-Castro,

    1. IPATIMUP—Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
    2. FMUP—Faculty of Medicine, University of Porto, Porto, Portugal
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  • Ângela M. Sousa Costa,

    1. IPATIMUP—Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
    2. FMUP—Faculty of Medicine, University of Porto, Porto, Portugal
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  • Maria José Oliveira,

    1. FMUP—Faculty of Medicine, University of Porto, Porto, Portugal
    2. INEB—Institute for Biomedical Engineering, Porto, Portugal
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  • Inês Barbosa,

    1. IPATIMUP—Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
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  • Ana Sofia Rocha,

    1. IPATIMUP—Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
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  • Luisa Azevedo,

    1. IPATIMUP—Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
    2. FCUP—Faculty of Sciences, University of Porto, Porto, Portugal
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  • Luís Teixeira da Costa

    Corresponding author
    1. IPATIMUP—Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
    2. FMUP—Faculty of Medicine, University of Porto, Porto, Portugal
    3. ICAAM—Institute of Agricultural and Environmental Mediterranean Sciences, University of Évora, Évora, Portugal
    • Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Núcleo da Mitra, Apartado 94, 7002-774 Évora, Portugal.
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Abstract

NET family members have recently emerged as important players in the development of multiple structures, from the trachea of fly larvae to the vertebrate eye and human breast cancers. However, their mechanisms of action are still poorly understood, and we lack a detailed characterization of their functional domains, as well as gene expression patterns—particularly in adult mammals. Here, we present a characterization of human NLZ1/ZNF703 (NocA-like zinc finger 1/Zinc finger 703), one of the two human NET family member genes. We show that the gene is ubiquitously expressed in adult human and mouse tissues, that three mRNA species with the same coding sequence are generated by alternative polyadenylation, and that the encoded protein contains six evolutionarily conserved domains, three of which are specific to NET proteins. Finally, we present functional evidence that these domains are necessary for proper subcellular distribution of and transcription repression by the NLZ1 protein, but not for its interaction with Groucho family co-repressors. J. Cell. Biochem. 114: 120–133, 2012. © 2012 Wiley Periodicals, Inc.

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