• Please log in or register to access this feature.

Article

You have free access to this content

Differentiation potential and GFP labeling of sheep bone marrow-derived mesenchymal stem cells

  1. Marta Czernik1,*,
  2. Antonella Fidanza1,
  3. Martina Sardi1,
  4. Cesare Galli2,3,4,
  5. Dario Brunetti2,†,
  6. Daniela Malatesta1,
  7. Leonardo Della Salda1,
  8. Kazutsugu Matsukawa5,
  9. Grazyna E. Ptak1,
  10. Pasqualino Loi1,*

Article first published online: 14 NOV 2012

DOI: 10.1002/jcb.24310

Issue

Journal of Cellular Biochemistry

Journal of Cellular Biochemistry

Volume 114, Issue 1, pages 134–143, January 2013

Additional Information

How to Cite

Czernik, M., Fidanza, A., Sardi, M., Galli, C., Brunetti, D., Malatesta, D., Della Salda, L., Matsukawa, K., Ptak, G. E. and Loi, P. (2013), Differentiation potential and GFP labeling of sheep bone marrow-derived mesenchymal stem cells. J. Cell. Biochem., 114: 134–143. doi: 10.1002/jcb.24310

Author Information

  1. 1

    Department of Comparative Biomedical Science, University of Teramo, 64100 Teramo, Italy

  2. 2

    Laboratory of Reproductive Technologies, Avantea Srl, 26100, Cremona, Italy

  3. 3

    Department of Veterinary Medical Sciences, University di Bologna, Ozzano Emilia, Italy

  4. 4

    Fondazione Avantea, 26100 Cremona, Italy

  5. 5

    Laboratory of Animal Science, College of Agriculture, Kochi University, Nankoku, Japan

  1. †

    Division of Molecular Neurogenetics, IRCCS Foundation Neurological Institute, “C.Besta” Via Temolo 4, 20126 Milano, Italy

*Department of Comparative Biomedical Science, University of Teramo, Piazza Aldo Morro 45, 64100 Teramo, Italy.

Publication History

  1. Issue published online: 14 NOV 2012
  2. Article first published online: 14 NOV 2012
  3. Accepted manuscript online: 6 AUG 2012 09:23AM EST
  4. Manuscript Accepted: 26 JUL 2012
  5. Manuscript Received: 29 JUN 2012

Funded by

  • The European Research Council. Grant Number: FP7/2007-2013
  • Programme IDEAS. Grant Number: 210103
  • EU FP6 (Xenome). Grant Number: LSHB-CT-2006-037377
  • EU FP7 (Plurisys). Grant Number: 223485
  • Regione Lombardia (Superpig Project) in CG Laboratory
  • FP7 EU “NextGen,” PRIN 2009 MIUR, and Tercas

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (462K)

REFERENCES

  • Abdallah BM, Kassem M. 2008. Human mesenchymal stem cells: from basic biology to clinical applications. Gen Ther 15:109–116.
  • Baertschiger RM, Serre-Beinier V, Morel P, Bosco D, Peyrou M, Clément S, Sgroi A, Kaelin A, Buhler LH, Gonelle-Gispert C. 2009. Fibrogenic potential of human multipotent mesenchymal stromal cells in injured liver. PLoS ONE 17:e6657.
  • Bianco P, Robey PG. 2001. Stem cells in tissue engineering. Nature 414:118–121.
  • Brunetti D, Perota I, Lagutina S, Colleoni R, Duchi F, Calabrese M, Seveso E, Cozzi G, Lazzari F, Lucchini L, Galli C. 2008. Transgene expression of green fluorescent protein and germ line transmission in cloned pigs derived from in vitro transfected adult fibroblasts. Cloning Stem Cells 10:409–419.
  • Caplan AI. 1991. Mesenchymal stem cells. J Orthop Res 9:641–650.
    Direct Link:
  • Caplan AI. 2007. Adult stem cells for tissue engineering versus regenerative medicine. J Cell Physiol 213:341–347.
  • Colleoni S, Donofrio G, Lagutina I, Duchi R, Galli C, Lazzari G. 2005. Establishment, differentiation, electroporation, viral transduction, and nuclear transfer of bovine and porcine mesenchymal stem cells. Cloning Stem Cells 7:154–166.
  • Friedenstein AJ, Chailakhyan RK, Gerasimov UV. 1987. Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet 203:263–272.
  • Galmozzi E, Facchetti F, La Porta CA. 2006. Cancer stem cells and therapeutic perspectives. Curr Med Chem 13:603–607.
  • Howlett CR, Cavé J, Williamson M, Farmer J, Ali SY, Bab I, Owen ME. 1986. Mineralization in vitro cultures of rabbit marrow stromal cells. Clin Orthop Relat Res 213:251–263.
  • Jeong BK, Sebastiano V, Wu G, Araúzo-Bravo PS, Gentile L, Ko K, Ruau D, Ehrich M, Van den Boom E, Meyer J, Hübner K, Bernemann C, Ortmeier C, Zenke M, Fleischmann BK, Zaehres H, Schöler HR. 2009. Oct4-induced pluripotency in adult neural stem cells. Cell 136:411–419.
  • Johnson A, Dorshkind K. 1986. Stromal cells in myeloidlymphoid long-term bone marrow cultures can support multiple hemopoietic lineages modulate their production of hemopoietic growth factors. Blood 68:1348–1354.
  • Kadiyala S, Young RG, Thiede MA, Bruder SP. 1997. Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro. Cell Transplant 6:125–134.
  • Kadouchi I, Sakamoto K, Tangjiao L, Murakami T, Kobayashi E, Hoshino Y, Yamaguchi A. 2009. Latexin is involved in bone morphogenetic protein-2-induced chondrocyte differentiation. Biochem Biophys Res Commun 378:600–604.
  • Kawaguchi J. 2006. Embryonic stem cell protocols. Volume 2: differentiation models. In: Turksen K, editors. 2nd edition. Humana Press Inc. 144 p. Ottawa Health Research Institute, Ottawa Ontario Canada, Series: Methods in Molecular Biology, Volume No. 330.
  • Krebsbach PH, Mankani MH, Satomura K, Kuznetsov SA, Robey PG. 1998. Repair of craniotomy defects using bone marrow stromal cells. Transplantation 27:1272–1278.
  • Kuznetsov SA, Krebsbach PH, Satomura K, Kerr J, Riminucci M, Benayahu D, Robey PG. 1997. Single-colony derived strains of human marrow stromal fibroblasts form bone after transplantation in vivo. J Bone Miner Res 9:1335–1347.
  • Lublin FD, Knobler RL, Doherty PC. 1989. Relapsing experimental allergic encephalomyelitis in radiation bone marrow chimars between high and low susceptible strains of mice. Clin Exp Immunol 66:491–496.
  • Martin PJ, Antin JH, Weisdorf DJ, Paton V, Horowitz MM. 2002. Reporting of adverse event data in hematopoietic stem cell transplantation clinical trials involving investigational new drugs or devices: a report from the William Guy Forbeck Foundation 2001 focus meeting on clinical trials in hematopoietic stem cell transplantation Biol Blood Marrow Transplant 8:295–302.
  • McCarty RC, Gronthos S, Zannettino AC, Foster BK, Xian CJ. 2009. Characterisation and developmental potential of ovine bone morrow derived mesenchymal stem cells. Cell Physiol 219:324–333.
    Direct Link:
  • Niwa H, Yamamua K, Miyazaki J. 1991. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193–199.
  • Phinney DG, Prockop DJ. 2007. Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation modes of tissue repair-current views. Stem Cells 25:2896–2902.
  • Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. 1999. Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147.
  • Pittenger MF, Mosca JD, McIntosh KR. 2000. Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma. Curr Top Microbiol Immunol 251:3–11.
  • Pittenger MF. Mesenchymal stem cells from adult bone marrow. 2008. Methods Mol Biol 449:27–44.
  • Ponticiello MS, Schinagl RM, Kadiyala S, Barry FP. 2000. Gelatin-based resorbable sponge as a carrier matrix for human mesenchymal stem cells in cartilage regeneration therapy. J Biomed Mater Res 52(2):246–255.
    Direct Link:
  • Prockop DJ. 2009. Repair of tissues by adult stem/progenitor cells (MSCs): controversies, myths, and changing paradigms. Mol Ther 17:939–946.
  • Procop DJ. 1997. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276:71–74.
  • Ptak G, Loi P, Dattena M, Tishner M, Cappai P. 1999. Offspring from one month old lambs: studies on the developmental capability of prepubertal oocytes. Biol Reprod 61:1568–1574.
  • Ptak G, Clinton M, Tischner M, Barboni B, Mattioli M, Loi P. 2002. Improving delivery and offspring viability of in vitro-production and cloned sheep embryos. Biol Reprod 67:1719–1725.
  • Quarto R, Mastrogiacomo M, Cancedda R, Kutepov SM, Mukhachev V, Lavroukov A, Kon Marcacci E. 2001. Repair of large bone defects with the use of autolegous bone marrow stromal cells. N Engl J Med 344:385–386.
  • Quevedo HC, Hatzistergos KE, Oskouei BN, Feigenbaum GS, Rodriguez JE, Valdes D, Pattany PM, Zambrano JP, Hu Q, McNiece I, Heldman AW, Hare JM. 2009. Allogeneic mesenchymal stem cells restore cardiac function in chronic ischemic cardiomyopathy via trilineage differentiating capacity. Proc Natl Acad Sci USA 106:14022–14027.
  • Ringe J, Kaps C, Schmitt B, Büscher K, Bartel J, Smolian H, Schultz O, Burmester GR, Häupl T, Sittinger M. 2002. Porcine mesenchymal stem cells. Induction of distinct mesenchymal cell lineages. Cell Tissue Res 307:321–327.
  • Sell S. 2006. Cancer stem cells and differentiation. Therapy Tumour Biol 27:59–70.
  • Stief A, Winter DM, Stratling WH, Sippel AE. 1989. A nuclear DNA attachment element mediates elevated and position-independent gene activity. Nature 341:343–345.
  • Takahashi K, Yamanaka S. 2006. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676.
  • Terada N, Hamazaki T, Oka M, Hoki M, Mastalerz DM, Nakano Y, Meyer EM, Morel L, Petersen BE, Scott EW. 2002. Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion. Nature 414:542–545.
  • Toma C, Pittenger MF, Cahill KS, Byrne BJ, Kessler PD. 2002. Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart. Circulation 105:93–98.
  • Ying QL, Nichols J, Evans EP, Smith AG. 2002. Changing potency by spontaneous fusion. Nature 416:545–548.
View Full Article (HTML) Get PDF (462K)