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Glutamate-ammonia ligase and reduction of G0 population in PANC-1 cells

Authors

  • Jong-Ho Choi,

    1. College of Medicine, CHA University, CHA General Hospital, Gyeonggi-Do 463-840, Republic of Korea
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  • Key-Hwan Lim,

    1. Department of Biomedical Science, CHA University, CHA General Hospital, Gyeonggi-Do 463-840, Republic of Korea
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  • Eunmi Park,

    1. Division of Radiation Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115
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  • Ji-Young Kim,

    1. Department of Pathology, CHA University, CHA General Hospital, Seoul 135-080, Republic of Korea
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  • Young-Kil Choi,

    1. Department of Internal Medicine, CHA University, CHA General Hospital, Seoul 135-080, Republic of Korea
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  • Kwang-Hyun Baek

    Corresponding author
    1. College of Medicine, CHA University, CHA General Hospital, Gyeonggi-Do 463-840, Republic of Korea
    2. Department of Biomedical Science, CHA University, CHA General Hospital, Gyeonggi-Do 463-840, Republic of Korea
    3. Division of Radiation Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115
    • Department of Biomedical Science, CHA University, 502 Yatap-Dong, Bundang-Gu, Seongnam-Si, Gyeonggo-Do 463-840, Republic of Korea.
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Abstract

In our previous study, we screened and isolated genes that were up-regulated after partial pancreatectomy using transcriptomic analysis and glutamate-ammonia ligase (GLUL) was selected for further study based on its effect on differentiation and proliferation. In the immunohistochemical analysis, GLUL was highly up-regulated in the acinar cells and the ductal cells in the pancreas damaged through partial pancreatectomy. Overexpression of GLUL enhanced the proliferation of PANC-1 cells and INS-1 cells. GLUL overexpression shifted the major population of PANC-1 cells from the G0/G1 phase to G2/M phase. In the double thymidine blocking analysis, similar cycle duration was observed between mock cells and GLUL-overexpressing cells while GLUL-overexpressing cells were partially resistant to thymidine blocking. In the FACS analysis of cells stained with Pyronin Y and Hoechst 33342, GLUL-overexpressing cells showed lower population of cells in the G0-quiescent phase than mock cells (5–12%). In addition, GLUL-overexpressing cells had high activation levels of AKT, ERK1/2, JNK, PCNA, c-FOS, and P70S6K in PANC-1 cells. Taken together, these results suggest that GLUL contributes to pancreatic regeneration. J. Cell. Biochem. 114: 303–313, 2013. © 2012 Wiley Periodicals, Inc.

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