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Journal of Cellular Biochemistry

The effect of rotative stress on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts

Authors

  • Zhang Qing Hong,

    1. Department of Prosthodontics, Stomatology Hospital, College of Medical Sciences, Zhejiang University, No.395 Yan-an Road, Hangzhou 310006, China
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  • Liu Meng Tao,

    Corresponding author
    1. Department of Prosthodontics, Hengha Stomatology Hospital, No.20 Gu-dun Road, Hangzhou 310013, China
    • Department of Prosthodontics, Hengha Stomatology Hospital, No.20 Gu-dun Road, Hangzhou 310013, China.
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  • Zhou Yi,

    1. Department of Prosthodontics, Stomatology Hospital, College of Medical Sciences, Zhejiang University, No.395 Yan-an Road, Hangzhou 310006, China
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  • Liu Wei,

    1. Department of Prosthodontics, Stomatology Hospital, College of Medical Sciences, Zhejiang University, No.395 Yan-an Road, Hangzhou 310006, China
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  • Shen Ju Xiang,

    1. Department of Prosthodontics, Stomatology Hospital, College of Medical Sciences, Zhejiang University, No.395 Yan-an Road, Hangzhou 310006, China
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  • Liu Li

    1. Department of Prosthodontics, Stomatology Hospital, College of Medical Sciences, Zhejiang University, No.395 Yan-an Road, Hangzhou 310006, China
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Abstract

This study was designed to explore the effects of rotative stress on carbonic anhydrase II (CAII), TNF receptor superfamily member 6 (FAS), FAS ligand (FASL), osteoclast-associated receptor (OSCAR), and tartrate-resistant acid phosphatase (TRAP) gene expression in osteoclasts. Osteoclasts were induced from RAW264.7 cells cultured in medium containing recombinant murine soluble receptor activator of NF-Kβ ligand (sRANKL). The mRNA and protein expression of CAII, FAS, FASL, OSCAR, and TRAP genes in osteoclasts was detected by RT-PCR and Western blot, respectively, after osteoclasts were loaded at various rotative stress strengths and times. No significant differences in mRNA and protein expression were observed between any of the control groups (P > 0.05). Importantly, rotative stress had a significant effect on the mRNA and protein expression of these genes (P < 0.05). We found a negative relationship between rotative stress strength and prolonged loading time and the expression of FAS/FASL genes in osteoclasts. In addition, there was a positive relationship between rotative stress strength and prolonged loading time and the expression of CAII, OSCAR, or TRAP genes in osteoclasts. Based on these results, rotative stress has a significant effect on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts. J. Cell. Biochem. 114: 388–397, 2013. © 2012 Wiley Periodicals, Inc.

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