All authors state that they have no conflicts of interest.
Article first published online: 17 DEC 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 114, Issue 2, pages 471–479, February 2013
How to Cite
Zhang, Y. and Ross, A. C. (2013), Retinoic acid and the transcription factor MafB act together and differentially to regulate aggrecan and matrix metalloproteinase gene expression in neonatal chondrocytes. J. Cell. Biochem., 114: 471–479. doi: 10.1002/jcb.24387
Materials disclosure: Information about the materials and methods used is available from Dr. Ross. No permanent new materials or models were generated.
Author's contribution: Study design, conduct, data interpretation, and manuscript preparation: Y.Z. and A.C.R. A.C.R. takes responsibility for the integrity of the data analysis.
- Issue published online: 17 DEC 2012
- Article first published online: 17 DEC 2012
- Accepted manuscript online: 7 SEP 2012 07:54AM EST
- Manuscript Accepted: 30 AUG 2012
- Manuscript Received: 21 JUN 2012
- NIH. Grant Numbers: DK41479, HD 066982, CA90214
- RETINOIC ACID;
- MATRIX METALLOPROTEINASE
Vitamin A (VA) and its active form, retinoic acid (RA), are regulators of skeletal development and chondrogenesis. MafB, a transcription factor, has been identified as an important mediator in monocyte and osteoclast differentiation. However, the presence and function of MafB in chondrocytes is not clear. In this study, MafB gene expression was regulated by both the VA status of the mother (VA-marginal, adequate, and supplemented diets) and by direct oral supplementation of the neonates with VARA (VA mixed with 10% RA). Expression was highest in neonates of VA-supplemented versus VA-marginal dams (P < 0.05), and in VARA-treated versus placebo-treated neonates across all diet groups (P < 0.05). To examine cellular changes, primary chondrocytes derived from neonatal rat ribs were cultured in the presence of RA for up to 48 h. MafB mRNA exhibited a time- and dose-dependent increase in response to RA, while the induction of MafB mRNA was attenuated by BMS-493, a pan-RAR inverse agonist, implicating RAR signaling in the regulation of MafB. The genetic knockdown of MafB in chondrocytes using siRNA (MafBSI chondrocytes) abrogated the RA-induced increase in MafB expression. MafBSI chondrocytes expressed higher levels of aggrecan mRNA. Additionally, the increased matrix metalloproteinase (MMP)3 and MMP13 gene expression due to RA was attenuated in MafBSI chondrocytes, while total extracellular matrix staining was increased. These results support a role for MafB as a regulator of chondrocyte gene expression and matrix formation via control of aggrecan, MMP3 and MMP13 expression, and indicate an important role for RA in the regulation of MafB. J. Cell. Biochem. 114: 471–479, 2013. © 2012 Wiley Periodicals, Inc.