The authors declared they have no conflict of interest.
Tunneling nanotube formation is essential for the regulation of osteoclastogenesis†
Article first published online: 16 APR 2013
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 114, Issue 6, pages 1238–1247, June 2013
How to Cite
Takahashi, A., Kukita, A., Li, Y.-j., Zhang, J.-q., Nomiyama, H., Yamaza, T., Ayukawa, Y., Koyano, K. and Kukita, T. (2013), Tunneling nanotube formation is essential for the regulation of osteoclastogenesis. J. Cell. Biochem., 114: 1238–1247. doi: 10.1002/jcb.24433
- Issue published online: 16 APR 2013
- Article first published online: 16 APR 2013
- Accepted manuscript online: 5 NOV 2012 12:13PM EST
- Manuscript Accepted: 22 OCT 2012
- Manuscript Received: 15 OCT 2012
- Grant-in-Aid for Scientific Research (Scientific Reseach B)
- Challenging Exploratory Research
- Japan Society for Promotion of Science (JSPS). Grant Numbers: 21659424, 21390492
- MEMBRANE NANOTUBE;
- OSTEOCLAST DIFFERENTIATION;
- M-Sec GENE;
- TIME-LAPSE ANALYSIS;
- GFP-FUSION PROTEIN;
- MEMBRANE PHOSPHOLIPID
Osteoclasts are the multinucleated giant cells formed by cell fusion of mononuclear osteoclast precursors. Despite the finding of several membrane proteins involving DC-STAMP as regulatory proteins required for fusion among osteoclast precursors, cellular and molecular events concerning this process are still ambiguous. Here we identified Tunneling Nanotubes (TNTs), long intercellular bridges with small diameters, as the essential cellular structure for intercellular communication among osteoclast precursors in prior to cell fusion. Formation of TNTs was highly associated with osteoclastogenesis and it was accompanied with the significant induction of the M-Sec gene, an essential gene for TNT formation. M-Sec gene expression was significantly upregulated by RANKL-treatment in osteoclast precursor cell line. Blockage of TNT formation by Latrunclin B or by M-Sec siRNA significantly suppressed osteoclastogenesis. We have detected the rapid intercellular transport of not only the membrane phospholipids labeled with DiI but also the DC-STAMP-GFP fusion protein through TNTs formed among osteoclast precursors during osteoclastogenesis. Transportation of such regulatory molecules through TNTs would be essential for the process of the specific cell fusion among osteoclast precursors. J. Cell. Biochem. 114: 1238–1247, 2013. © 2012 Wiley Periodicals, Inc.