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Journal of Cellular Biochemistry

Human adipose CD34+CD90+ stem cells and collagen scaffold constructs grafted in vivo fabricate loose connective and adipose tissues

Authors

  • Giuseppe A. Ferraro,

    1. Dipartimento di Scienze Ortopediche, Riabilitative, Traumatologiche e Plastico-Ricostruttive, Seconda Università degli Studi di Napoli, Italy
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  • Francesco De Francesco,

    1. Dipartimento di Scienze Ortopediche, Riabilitative, Traumatologiche e Plastico-Ricostruttive, Seconda Università degli Studi di Napoli, Italy
    2. Dipartimento di Medicina Sperimentale, Sezione di Istologia ed Embriologia Medica, Tissue Engineering and Regenerative Laboratory (TERM), Seconda Università degli Studi di Napoli, Italy
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  • Gianfranco Nicoletti,

    1. Dipartimento di Scienze Ortopediche, Riabilitative, Traumatologiche e Plastico-Ricostruttive, Seconda Università degli Studi di Napoli, Italy
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  • Francesca Paino,

    1. Dipartimento di Medicina Sperimentale, Sezione di Istologia ed Embriologia Medica, Tissue Engineering and Regenerative Laboratory (TERM), Seconda Università degli Studi di Napoli, Italy
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  • Vincenzo Desiderio,

    1. Dipartimento di Medicina Sperimentale, Sezione di Istologia ed Embriologia Medica, Tissue Engineering and Regenerative Laboratory (TERM), Seconda Università degli Studi di Napoli, Italy
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  • Virginia Tirino,

    Corresponding author
    1. Dipartimento di Medicina Sperimentale, Sezione di Istologia ed Embriologia Medica, Tissue Engineering and Regenerative Laboratory (TERM), Seconda Università degli Studi di Napoli, Italy
    • Section of Histology and Embryology, Faculty of Medicine and Surgery, Department of Experimental Medicine, 5 via L. Armanni, Naples 80138, Italy.
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  • Francesco D'Andrea

    1. Dipartimento di Scienze Ortopediche, Riabilitative, Traumatologiche e Plastico-Ricostruttive, Seconda Università degli Studi di Napoli, Italy
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  • The authors declare no potential conflicts of interest.

Abstract

Stem cell based therapies for the repair and regeneration of various tissues are of great interest for a high number of diseases. Adult stem cells, instead, are more available, abundant and harvested with minimally invasive procedures. In particular, mesenchymal stem cells (MSCs) are multi-potent progenitors, able to differentiate into bone, cartilage, and adipose tissues. Human adult adipose tissue seems to be the most abundant source of MSCs and, due to its easy accessibility; it is able to give a considerable amount of stem cells. In this study, we selected MSCs co-expressing CD34 and CD90 from adipose tissue. This stem cell population displayed higher proliferative capacity than CD34CD90 cells and was able to differentiate in vitro into adipocytes (PPARγ+ and adiponectin+) and endothelial cells (CD31+VEGF+Flk1+). In addition, in methylcellulose without VEGF, it formed a vascular network. The aim of this study was to investigate differentiation potential of human adipose CD34+/CD90+ stem cells loaded onto commercial collagen sponges already used in clinical practice (Gingistat) both in vitro and in vivo. The results of this study clearly demonstrate that human adult adipose and loose connective tissues can be obtained in vivo, highlighting that CD34+/CD90 ASCs are extremely useful for regenerative medicine. J. Cell. Biochem. 114: 1039–1049, 2013. © 2012 Wiley Periodicals, Inc.

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