The authors declared they have no conflict of interest.
Osteogenesis and expression of the bone marrow niche in endothelial cell-depleted HipOPs†
Article first published online: 14 MAR 2013
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 114, Issue 5, pages 1066–1073, May 2013
How to Cite
McKenzie, K. P., Mayer, D. C. and Aubin, J. E. (2013), Osteogenesis and expression of the bone marrow niche in endothelial cell-depleted HipOPs. J. Cell. Biochem., 114: 1066–1073. doi: 10.1002/jcb.24446
- Issue published online: 14 MAR 2013
- Article first published online: 14 MAR 2013
- Accepted manuscript online: 13 NOV 2012 08:46AM EST
- Manuscript Accepted: 30 OCT 2012
- Manuscript Received: 25 OCT 2012
- Canadian Institutes of Health Research (CIHR). Grant Number: FRN 83704
- The Edward Dunlop Ontario Graduate Scholarship in Science and Technology
- Canadian Arthritis Network Trainee Award
- Skeletal Regenerative Medicine Team (CIHR Team) Studentship
- Life Science Undergraduate Research Award
- VASCULAR ENDOTHELIAL CELLS;
- MESENCHYMAL STEM CELLS;
- HEMATOPOIETIC STEM CELL NICHE;
The identification and purification of murine multipotent mesenchymal stem cells (MSCs) have been difficult due to their low frequency, the presence of contaminating cell types and lack of unambiguous markers. Using a magnetic micro-beads negative selection technique to remove hematopoietic cells from mouse bone marrow stromal cells (BMSCs), our lab recently isolated a highly purified osteoprogenitor (HipOP) population that was also enriched for other mesenchymal precursors, including MSCs [Itoh and Aubin, 2009]. We now report that HipOPs are also highly enriched in vascular endothelial cells (VECs), which we hypothesized were an accessory cell type regulating osteogenesis. However, when VECs were immunodepleted from HipOPs with anti-CD31 antibodies, the resulting CD31(−) HipOP population had equal osteogenic capacity to the HipOPs in vitro and in vivo. Analysis of gene expression of Ncad, Pth1r, Ang1, Cxcl12, Jag1, Pdgfr-β, α-sma, Desmin, and Ng2 suggested that both HipOPs and CD31(−) HipOPs are hemopoietic stem cell (HSC) niche populations. However, the data support the view that osteoblast differentiation and depletion of VECs modulate the HSC niche. J. Cell. Biochem. 114: 1066–1073, 2013. © 2012 Wiley Periodicals, Inc.