Journal of Cellular Biochemistry

Anti-adhesive property of P-selectin glycoprotein ligand-1 (PSGL-1) due to steric hindrance effect

Authors

  • Saori Umeki,

    1. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan
    2. Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
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  • Ryoichi Suzuki,

    1. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan
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  • Yasuo Ema,

    1. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan
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  • Masayuki Shimojima,

    1. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan
    2. Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
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  • Yorihiro Nishimura,

    1. Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan
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  • Masaru Okuda,

    1. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan
    2. Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
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  • Takuya Mizuno

    Corresponding author
    1. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan
    2. Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
    • Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
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  • The authors declare that they have no conflict of interest.

Abstract

P-selectin glycoprotein ligand-1 (PSGL-1) is an adhesive molecule that is known to be a ligand for P-selectin. An anti-adhesive property of PSGL-1 has not been previously reported. In this study, we show that PSGL-1 expression is anti-adhesive for adherent cells and we have elucidated the underlying mechanism. Overexpression of PSGL-1 induced cell rounding and floating in HEK293T cells. Similar phenomena were demonstrated in other adherent cell lines with overexpression of PSGL-1. PSGL-1 overexpression inhibits access of antibodies to cell surface molecules such as integrins, HLA and CD25. Cells transfected with PSGL-1 deletion mutants that lack a large part of the extracellular domain and chimeric construct expressing extracellular CD86 and intracellular PSGL-1 only showed rounded morphology, but there are no floating cells. These results indicated that PSGL-1 causes steric hindrance due to the extended structure of its extracellular domain that is highly O-glycosylated, but intracellular domain also has some effect on cell rounding. This study implies that PSGL-1 has Janus-faced functions, being both adhesive and anti-adhesive. J. Cell. Biochem. 114: 1271–1285, 2013. © 2013 Wiley Periodicals, Inc.

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