Journal of Cellular Biochemistry

MiR-29 mediates TGFβ1-induced extracellular matrix synthesis through activation of PI3K-AKT pathway in human lung fibroblasts

Authors

  • Tao Yang,

    1. National Engineering Laboratory for Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, People's Republic of China
    2. College of Chemistry and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan 410076, People's Republic of China
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  • Ying Liang,

    1. National Engineering Laboratory for Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, People's Republic of China
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  • Qinlu Lin,

    1. National Engineering Laboratory for Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, People's Republic of China
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  • Junwen Liu,

    1. Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, Changsha 410013, Hunan Province, People's Republic of China
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  • Feijun Luo,

    1. National Engineering Laboratory for Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, People's Republic of China
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  • Xinhua Li,

    1. Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, People's Republic of China
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  • Hui Zhou,

    1. National Engineering Laboratory for Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, People's Republic of China
    2. College of Chemistry and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan 410076, People's Republic of China
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  • Sheng Zhuang,

    1. National Engineering Laboratory for Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, People's Republic of China
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  • Hongliang Zhang

    Corresponding author
    1. Emergency Department, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, People's Republic of China
    • Emergency Department, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, People's Republic of China.
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  • Tao Yang, Ying Liang, Quinlu Lin, and Junwen Liu contributed equally to this work.

Abstract

TGFβ1 is very important in the synthesis and degradation of extracellular matrix, and also in the mediation of human lung fibroblasts proliferation, and miR-29 plays an important role in this process. To explore the interactions of miR-29 family members and TGFβ1, the effects of transforming growth factor TGFβ1 on the expression of miR-29 and whether miR-29 is involved in pro-survival signaling pathways mediated by TGFβ1 were examined in human lung fibroblasts. Treatment of the human embryonic lung fibroblast cell line IMR90 with TGFβ1 caused a decrease in expression of miR-29a/b/c by real-time PCR analysis. TGFβ1 stimulation increased cell proliferation, colony formation and up-regulated expression of COL1A1; transfecting with miR-29a/b/c mimics reverse TGFβ1-induced phenotype changes in IMR90 cells. Western blot analyses showed that TGFβ1 treatment unchanged total protein expression levels of PI3K or AKT, but the expression levels of p-PI3K, p-AKT, and COL1A1 were increased; and miR-19a/b/c mimics interfering blocked phosphorylation of PI3K or AKT and decreased expression of COL1A1 after TGFβ1 treatment. The results indicate that TGFβ1 beta uses the PI3k-Akt pathway in these embryonic fibroblasts and miR29 blocks this activation pathway. It indicates a novel biological function of the PI3K-Akt pathway in IMR90. Elevated expression of miR-29 may play an important role in the pathogenesis of diseases related to fibrogenic reactions in human lung fibroblasts. J. Cell. Biochem. 114: 1336–1342, 2013. © 2013 Wiley Periodicals, Inc.

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