Journal of Cellular Biochemistry

Tumor suppressor gene p16/INK4A/CDKN2A-dependent regulation into and out of the cell cycle in a spontaneous canine model of breast cancer

Authors

  • Payal Agarwal,

    1. Department of Pathobiology, College of Veterinary Medicine, AURIC—Auburn University Research Initiative in Cancer, Auburn, Albama 36849-5519
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  • Maninder Sandey,

    1. Department of Pathobiology, College of Veterinary Medicine, AURIC—Auburn University Research Initiative in Cancer, Auburn, Albama 36849-5519
    2. Scott-Ritchey Research Center, College of Veterinary Medicine, AURIC—Auburn University Research Initiative in Cancer, Auburn, Albama
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  • Patricia DeInnocentes,

    1. Department of Pathobiology, College of Veterinary Medicine, AURIC—Auburn University Research Initiative in Cancer, Auburn, Albama 36849-5519
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  • R. Curtis Bird

    Corresponding author
    1. Department of Pathobiology, College of Veterinary Medicine, AURIC—Auburn University Research Initiative in Cancer, Auburn, Albama 36849-5519
    • Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5519.
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  • Conflicts of interest: Nothing to declare.

Abstract

p16/INK4A/CDKN2A is an important tumor suppressor gene that arrests cell cycle in G1 phase inhibiting binding of CDK4/6 with cyclin D1, leaving the Rb tumor suppressor protein unphosphorylated and E2F bound and inactive. We hypothesized that p16 has a role in exit from cell cycle that becomes defective in cancer cells. Well characterized p16-defective canine mammary cancer cell lines (CMT28, CMT27, and CMT12), derived stably p16-transfected CMT cell clones (CMT27A, CMT27H, CMT28A, and CMT28F), and normal canine fibroblasts (NCF), were used to investigate expression of p16 after serum starvation into quiescence followed by re-feeding to induce cell cycle re-entry. The parental CMT cell lines used lack p16 expression either at the mRNA or protein expression levels, while p27 and other p16-associated proteins, including CDK4, CDK6, cyclin D1, and Rb, were expressed. We have successfully demonstrated cell cycle arrest and relatively synchronous cell cycle re-entry in parental CMT12, CMT28 and NCF cells as well as p16 transfected CMT27A, CMT27H, CMT28A, and CMT28F cells and confirmed this by 3H-thymidine incorporation and flow cytometric analysis of cell cycle phase distribution. p16-transfected CMT27A and CMT27H cells exited cell cycle post-serum-starvation in contrast to parental CMT27 cells. NCF, CMT27A, and CMT28F cells expressed upregulated levels of p27 and p16 mRNA, post-serum starvation, as cells exited cell cycle and entered quiescence. Because quiescence and differentiation are associated with increased levels of p27, our data demonstrating that p16 was upregulated along with p27 during quiescence, suggests a potential role for p16 in maintaining these non-proliferative states. J. Cell. Biochem. 114: 1355–1363, 2013. © 2012 Wiley Periodicals, Inc.

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