Get access
Journal of Cellular Biochemistry

Overexpression of microRNA-122 enhances in vitro hepatic differentiation of fetal liver-derived stem/progenitor cells

Authors

  • Ravi Doddapaneni,

    1. Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education & Research, Chandigarh 160012, India
    Search for more papers by this author
  • Yogesh K. Chawla,

    1. Department of Hepatology, Post Graduate Institute of Medical Education & Research, Chandigarh 160012, India
    Search for more papers by this author
  • Ashim Das,

    1. Department of Histopathology, Post Graduate Institute of Medical Education & Research, Chandigarh 160012, India
    Search for more papers by this author
  • Jasvinder Kaur Kalra,

    1. Department of Obstetrics & Gynaecology, Post Graduate Institute of Medical Education & Research, Chandigarh 160012, India
    Search for more papers by this author
  • Sujata Ghosh,

    1. Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education & Research, Chandigarh 160012, India
    Search for more papers by this author
  • Dr. Anuradha Chakraborti

    Corresponding author
    1. Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education & Research, Chandigarh 160012, India
    • Professor, Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education & Research, Chandigarh 160012, India.
    Search for more papers by this author

Abstract

MicroRNAs (miRNAs) are a versatile class of tiny non-coding RNAs involved in regulation of various biological processes. miRNA-122 (miR-122) is specifically and abundantly expressed in human liver. However, the role of miR-122 in differentiation of fetal liver stem/progenitor cells into hepatocytes remains unclear. In this study, dual positive CD34+/CD117+ expressing human fetal liver stem/progenitor cells was enriched by magnetic cell sorting and cultured in vitro. The level of miR-122 was found to be increased at specific time intervals. Interestingly, during the differentiation process of hepatocyte-like cells, the increase in expression of miR-122 was positively correlated with expression of hepatocyte-specific genes. The status of differentiation process was improved by transfection of miR-122 into enriched stem/progenitor cells. The expression level of hepatic-specific genes as well as liver-enriched transcription factors (LETFs) was significantly increased by overexpression of miR-122 in fetal liver stem/progenitor cells. Thus, the study delineated the role of hepato-specific miR-122 in differentiation of fetal liver stem/progenitor cells into hepatocyte-like cells which could be used as a therapeutic target molecule to generate abundant hepatocytes. J. Cell. Biochem. 114: 1575–1583, 2013. © 2013 Wiley Periodicals, Inc.

Get access to the full text of this article

Ancillary