Run Chen, Chun-Yan Duan, and Shao-Kun Chen contributed equally to this work.
Article first published online: 11 JUN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 114, Issue 8, pages 1789–1799, August 2013
How to Cite
Chen, R., Duan, C.-Y., Chen, S.-K., Zhang, C.-Y., He, T., Li, H., Liu, Y.-P. and Dai, R.-Y. (2013), The suppressive role of p38 MAPK in cellular vacuole formation. J. Cell. Biochem., 114: 1789–1799. doi: 10.1002/jcb.24522
The authors declare no conflicts of interest.
- Issue published online: 11 JUN 2013
- Article first published online: 11 JUN 2013
- Accepted manuscript online: 26 FEB 2013 08:37AM EST
- Manuscript Accepted: 12 FEB 2013
- Manuscript Received: 29 NOV 2012
- National Natural Science Foundation of China. Grant Number: 81000886
- New Century Excellent Talents in University. Grant Number: NCET-11-1058
- CELLULAR VACUOLES;
- p38 MAPK;
- PERINUCLEAR REGION
Vacuolization of the cytoplasm is one of the dramatic and frequently observed phenomena in various cell types. Cellular vacuoles occur spontaneously or via a wide range of inductive stimuli, but the molecular mechanism involved in this process remains largely unknown. In this study, we investigated the role of the p38 and JNK pathways in the formation of cytoplasmic vacuoles. We found that p38 and JNK agonist anisomycin abolishes spontaneous cytoplasmic vacuolization of HepG2 cells through p38 activation, but not through JNK activation. Importantly, blocking the activity of p38 or suppression the expression of p38 elicits cytoplasmic vacuoles formation in various cancer cells. Furthermore, cytoplasmic vacuoles induced by p38 blocking are derived from the perinuclear region. These observations provide direct evidence for a role of p38 signaling in regulating the formation of cytoplasmic vacuoles. J. Cell. Biochem. 114: 1789–1799, 2013. © 2013 Wiley Periodicals, Inc.