The authors declare that they have no conflicts of interest.
Article first published online: 11 JUN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 114, Issue 8, pages 1800–1809, August 2013
How to Cite
Yoon, J. H., La Cho, M., Choi, Y. J., Back, J. Y., Park, M. K., Lee, S. W., Choi, B. J., Ashktorab, H., Smoot, D. T., Nam, S. W., Lee, J. Y. and Park, W. S. (2013), Gastrokine 1 regulates NF-κB signaling pathway and cytokine expression in gastric cancers. J. Cell. Biochem., 114: 1800–1809. doi: 10.1002/jcb.24524
Author contributions: J.H.Y., M.L.C., and W.S.P. conceived and designed the experiments, J.H.Y., M.L.C., Y.J.C., J.Y.B., M.K.P., S.W.L., B.J.C., H.A., and D.T.S. performed the experiments, J.H.Y. and W.S.P. analyzed the data, H.A., D.T.S., S.W.N., and J.Y.L. contributed reagents/materials/analysis tools, and W.S.P. and M.L.C. wrote the paper.
- Issue published online: 11 JUN 2013
- Article first published online: 11 JUN 2013
- Accepted manuscript online: 26 FEB 2013 08:37AM EST
- Manuscript Accepted: 13 FEB 2013
- Manuscript Received: 31 JAN 2013
- National Research Foundation of Korea (NRF). Grant Numbers: 2010-0020764, 2012R1A2A2A01002531
- GASTRIC CANCER;
- INFLAMMATORY MEDIATOR;
Gastrokine 1 (GKN1) plays an important role in the gastric mucosal defense mechanism and also acts as a functional gastric tumor suppressor. In this study, we examined the effect of GKN1 on the expression of inflammatory mediators, including NF-κB, COX-2, and cytokines in GKN1-transfected AGS cells and shGKN1-transfected HFE-145 cells. Lymphocyte migration and cell viability were also analyzed after treatment with GKN1 and inflammatory cytokines in AGS cells by transwell chemotaxis and an MTT assay, respectively. In GKN1-transfected AGS cells, we observed inactivation and reduced expression of NF-κB and COX-2, whereas shGKN1-transfected HFE-145 cells showed activation and increased expression of NF-κB and COX-2. GKN1 expression induced production of inflammatory cytokines including IL-8 and -17A, but decreased expression of IL-6 and -10. We also found IL-17A expression in 9 (13.6%) out of 166 gastric cancer tissues and its expression was closely associated with GKN1 expression. GKN1 also acted as a chemoattractant for the migration of Jurkat T cells and peripheral B lymphocytes in the transwell assay. In addition, GKN1 significantly reduced cell viability in both AGS and HFE-145 cells. These data suggest that the GKN1 gene may inhibit progression of gastric epithelial cells to cancer cells by regulating NF-κB signaling pathway and cytokine expression. J. Cell. Biochem. 114: 1800–1809, 2013. © 2013 Wiley Periodicals, Inc.