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Knock down of caveolin-1 affects morphological and functional hallmarks of human endothelial cells

Authors

  • Luca Madaro,

    1. Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy
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  • Fabrizio Antonangeli,

    1. Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy
    2. Faculty of Life Sciences, University of Manchester, Oxford Road, M13 9PT, Manchester, UK
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  • Annarita Favia,

    1. Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy
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  • Bianca Esposito,

    1. Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy
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  • Filippo Biamonte,

    1. Institute of Histology and Embryology, Faculty of Medicine, Catholic University of the Sacred Heart, Rome, Italy
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  • Marina Bouché,

    1. Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy
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  • Elio Ziparo,

    1. Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy
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  • Gigliola Sica,

    1. Institute of Histology and Embryology, Faculty of Medicine, Catholic University of the Sacred Heart, Rome, Italy
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  • Antonio Filippini,

    1. Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy
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  • Alessio D'Alessio

    Corresponding author
    1. Institute of Histology and Embryology, Faculty of Medicine, Catholic University of the Sacred Heart, Rome, Italy
    • Institute of Histology and Embryology, Catholic University of the Sacred Heart School of Medicine, Largo Francesco Vito 1, 00168 Rome, Italy.
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  • The authors have no financial conflicts of interest.

  • Luca Madaro and Fabrizio Antonangeli contributed equally to this work.

Abstract

Caveolin-1 (CAV1) is the principal structural component of caveolae which functions as scaffolding protein for the integration of a variety of signaling pathways. In this study, we investigated the involvement of CAV1 in endothelial cell (EC) functions and show that siRNA-induced CAV1 silencing in the human EC line EA.hy926 induces distinctive morphological changes, such as a marked increase in cell size and formation of stress fibers. Design-based stereology was employed in this work to make unbiased quantification of morphometric properties such as volume, length, and surface of CAV1 silenced versus control cells. In addition, we showed that downregulation of CAV1 affects cell cycle progression at G1/S phase transition most likely by perturbation of AKT signaling. With the aim to assess the contribution of CAV1 to typical biological processes of EC, we report here that CAV1 targeting affects cell migration and matrix metalloproteinases (MMPs) activity, and reduces angiogenesis in response to VEGF, in vitro. Taken together our data suggest that the proper expression of CAV1 is important not only for maintaining the appropriate morphology and size of ECs but it might represent a prospective molecular target for studying key biological mechanisms such as senescence and tumorigenesis. J. Cell. Biochem. 114: 1843–1851, 2013. © 2013 Wiley Periodicals, Inc.

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