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Journal of Cellular Biochemistry

Heat and radiofrequency plasma glow discharge pretreatment of a titanium alloy: Eveidence for enhanced osteoinductive properties

Authors

  • Bruce E. Rapuano,

    1. Hospital for Special Surgery Affiliated With the Weill Medical College of Cornell University, New York, New York 10021
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  • Herman Singh,

    1. Hospital for Special Surgery Affiliated With the Weill Medical College of Cornell University, New York, New York 10021
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  • Adele L. Boskey,

    1. Hospital for Special Surgery Affiliated With the Weill Medical College of Cornell University, New York, New York 10021
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  • Stephen B. Doty,

    1. Hospital for Special Surgery Affiliated With the Weill Medical College of Cornell University, New York, New York 10021
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  • Daniel E. MacDonald

    Corresponding author
    1. Hospital for Special Surgery Affiliated With the Weill Medical College of Cornell University, New York, New York 10021
    2. General Medical Research, James J. Peters VA Medical Center, Bronx, New York 10468
    3. Langmuir Center for Colloids and Interfaces, Columbia University, New York, New York 10027
    • Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021.
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Errata

This article is corrected by:

  1. Errata: Erratum: Heat and radiofrequency plasma glow discharge pretreatment of a titanium alloy: Evidence for enhanced osteoinductive properties Volume 114, Issue 9, 2201, Article first published online: 3 July 2013

  • The authors declare that they have no conflict of interest.

Abstract

It is believed that orthopedic and implant longevity can be improved by optimizing fixation, or direct bone-implant contact, through the stimulation of new bone formation around the implant. The purpose of this study was to determine whether heat (600°C) or radiofrequency plasma glow discharge (RFGD) pretreatment of Ti6Al4V stimulated calcium-phosphate mineral formation in cultures of attached MC3T3 osteoprogenitor cells with or without a fibronectin coating. Calcium-phosphate mineral was analyzed by flame atomic absorption spectrophotometry, scanning electron microscopy (SEM)/electron dispersive X-ray microanalysis (EDAX) and Fourier transformed infrared spectroscopy (FTIR). RFGD and heat pretreatments produced a general pattern of increased total soluble calcium levels, although the effect of heat pretreatment was greater than that of RFGD. SEM/EDAX showed the presence of calcium-and phosphorus-containing particles on untreated and treated disks that were more numerous on fibronectin-coated disks. These particles were observed earliest (1 week) on RFGD-pretreated surfaces. FTIR analyses showed that the heat pretreatment produced a general pattern of increased levels of apatite mineral at 2–4 weeks; a greater effect was observed for fibronectin-coated disks compared to uncoated disks. The observed findings suggest that heat pretreatment of Ti6Al4V increased the total mass of the mineral formed in MC3T3 osteoprogenitor cell cultures more than RFGD while the latter pretreatment hastened the early deposition of mineral. These findings help to support the hypothesis that the pretreatments enhance the osteoinductive properties of the alloy. J. Cell. Biochem. 114: 1917–1927, 2013. © 2013 Wiley Periodicals, Inc.

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