AKT primes snail-induced EMT concomitantly with the collective migration of squamous cell carcinoma cells

Authors

  • Gaku Okui,

    1. Department of Oral and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
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  • Kei Tobiume,

    Corresponding author
    • Department of Oral and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
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  • Andra Rizqiawan,

    1. Department of Oral and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
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  • Kazuhiro Yamamoto,

    1. Department of Oral and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
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  • Hideo Shigeishi,

    1. Department of Oral and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
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  • Shigehiro Ono,

    1. Department of Oral and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
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  • Koichiro Higashikawa,

    1. Department of Oral and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
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  • Nobuyuki Kamata

    Corresponding author
    • Department of Oral and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
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  • The authors declare no conflict of interest.

Correspondence to: Dr. Kei Tobiume and Nobuyuki Kamata, Department of Oral and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.

E-mail: tobi5651@hiroshima-u.ac.jp nokam@hiroshima-u.ac.jp

Abstract

In this study, we found that wounding of a confluent monolayer of squamous cell carcinoma (SCC) cells induced epithelial–mesenchymal transition (EMT) specifically at the edge of the wound. This process required the combined stimulation of TGFβ, TNFα, and PDGF-D. Such a combined cytokine treatment of confluent monolayers of the cells upregulated the expression levels of Snail and Slug via PI3K. The PI3K downstream effector, AKT, was dispensable for the upregulation of Snail and Slug, but essential for enabling EMT in response to upregulation of Snail and Slug. J. Cell. Biochem. 114: 2039–2049, 2013. © 2013 Wiley Periodicals, Inc.

Ancillary