The authors declare no conflict of interest.
AKT primes snail-induced EMT concomitantly with the collective migration of squamous cell carcinoma cells
Article first published online: 18 JUL 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 114, Issue 9, pages 2039–2049, September 2013
How to Cite
Okui, G., Tobiume, K., Rizqiawan, A., Yamamoto, K., Shigeishi, H., Ono, S., Higashikawa, K. and Kamata, N. (2013), AKT primes snail-induced EMT concomitantly with the collective migration of squamous cell carcinoma cells. J. Cell. Biochem., 114: 2039–2049. doi: 10.1002/jcb.24545
- Issue published online: 18 JUL 2013
- Article first published online: 18 JUL 2013
- Accepted manuscript online: 28 MAR 2013 11:39PM EST
- Manuscript Accepted: 13 MAR 2013
- Manuscript Received: 12 FEB 2013
- Ministry of Education, Culture, Sports, Science and Technology, Japan. Grant Numbers: 22249066, 21791823, 80363084
- SQUAMOUS CELL CARCINOMA;
In this study, we found that wounding of a confluent monolayer of squamous cell carcinoma (SCC) cells induced epithelial–mesenchymal transition (EMT) specifically at the edge of the wound. This process required the combined stimulation of TGFβ, TNFα, and PDGF-D. Such a combined cytokine treatment of confluent monolayers of the cells upregulated the expression levels of Snail and Slug via PI3K. The PI3K downstream effector, AKT, was dispensable for the upregulation of Snail and Slug, but essential for enabling EMT in response to upregulation of Snail and Slug. J. Cell. Biochem. 114: 2039–2049, 2013. © 2013 Wiley Periodicals, Inc.