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Journal of Cellular Biochemistry

The sumoylation of zinc-fingers and homeoboxes 1 (ZHX1) by ubc9 regulates its stability and transcriptional repression activity

Authors

  • Shuliang Chen,

    1. State Key Laboratory of Virology and the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China
    2. Biology Faculty of Hubei University, Wuhan, PR China
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  • Xiao Yu,

    1. State Key Laboratory of Virology and the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China
    2. Biology Faculty of Hubei University, Wuhan, PR China
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  • Quan Lei,

    1. State Key Laboratory of Virology and the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China
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  • Lixin Ma,

    Corresponding author
    • Biology Faculty of Hubei University, Wuhan, PR China
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  • Deyin Guo

    1. State Key Laboratory of Virology and the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China
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  • The authors declared they have no conflicts of interest.
  • Shuliang Chen and Xiao Yu contributed equally to this work.

Correspondence to: Lixin Ma, Biology Faculty of Hubei University, Wuhan 430062, PR China.

E-mail: malixin9@hotmail.com

Correspondence to: Deyin Guo, State Key Laboratory of Virology and the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

E-mail: dguo@whu.edu.cn

Abstract

Zinc-fingers and homeoboxes protein 1 (ZHX1) belongs to the ZF (zinc-fingers) class of homeodomain transcription factors, and its function remains largely unknown. ZHX1 has been previously found to interact with the activation domain of the nuclear factor Y subunit A (NFYA) and to have a transcriptional repression activity. Here, we report that the SUMO-E2 conjugating enzyme Ubc9 was identified to interact with ZHX1 by an interaction screen using a yeast two-hybrid system. This interaction was confirmed by co-immunoprecipitation and co-localization assays. Further study showed that ZHX1 is SUMOylated by Ubc9 with SUMO1 at the sites K159, K454, and K626. Furthermore, we demonstrated that the SUMOylation of ZHX1 regulated the stability, ubiquitination and transcriptional activity of ZHX1. J. Cell. Biochem. 114: 2323–2333, 2013. © 2013 Wiley Periodicals, Inc.

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